实验性急性胰腺炎大鼠肺内趋化因子基因的表达及其意义

为探讨急性坏死性胰腺炎 (ANP)大鼠模型肺组织单核细胞趋化性蛋白 1 (MCP 1 )mRNA的表达情况及其与肺损伤的关系 ,将 3 3只Wistar大鼠随机分为正常对照组和胰腺炎不同时间点 ( 1、4、1 2和 2 4h)各组 ,应用 3 .5 %牛磺胆酸钠逆行胰胆管注射制备ANP模型。采用RT PCR法检测肺组织MCP 1mRNA表达 ,同时测定肺组织湿 /干质量比率及病理改变。结果 :造模ANP 1h后肺组织MCP 1mRNA表达水平为 0 .40± 0 .0 8,较正常对照组表达水平( 0 .0 3± 0 .0 2 )明显增高 (P <0 .0 5 ) ,并持续升高至 2 4h( 1 .1 3± 0 .0 7) ,同时伴有肺组织病理损害 ,其严重程度与MCP 1mRNA表达及肺湿 /干质量比率与MCP 1mRNA表达均明显相关 (P <0 .0 5 )。提示 :大鼠ANP早期肺组织MCP 1即过度表达 ,MCP 1mRNA过度表达是ANP肺损害发生的原因之一 ,肺损伤严重程度与MCP 1mRNA表达的高低有关

Intrapulmonary Expression of Monocyte Chemotactic Protein-1 mRNA in Rats with Acute Necrotizing Pancreatitis

The aim was to investigate lung injury and intrapulmonary expression of MCP-1 mRNA in ANP. ANP was produced with 3.5% sodium taurocholate by the method of retrograde pancreatic-injection. Animals were sacrificed at 1,4,12 and 24 h after induction of pancreatitis. Intrapulmonary MCP-1 mRNA expression was assayed by RT-PCR. The following parameters were also measured: Serum lipases levels, pulmonary wet/dry weight ratio, histologic grading of AP and lung injury. Taurocholate pancreatitis showed necrosis, haemorrhage, and severe leucocyte infiltration in the pancreas, as well as alveolar septal thickening and leucocyte infiltration in the lung. There was intrapulmonary overexpression of the MCP-1 mRNA at the first hour and persisted up to the 24th h with the increase of serum lipases levels and pulmonary wet/dry weight ratio after pancreatitis induction. Expression of the MCP-1 mRNA correlated with score of the lung injury and pulmonary wet/dry weight ratio (P<0.05). The overexpression of MCP-1 mRNA is related to the production and severity of lung injury in ANP.