重组腺病毒载体局部介导CTLA4Ig延长小鼠异体移植皮肤的存活

目的 寻求一种简单可行的延长异体移植皮肤存活的方法。方法 将CTLA4Ig cDNA插入重组腺病毒载体柯氏质粒中，再通过同源重组构建CTLA如一重组腺病毒载体；观察该表达载体对体外培养的皮肤组织块的转染情况；并应用该载体转染皮肤移植物及局部创面，观察其对昆明小鼠→Balb c小鼠背部创面异体移植皮肤存活期的影响。结果 CTLA4Ig重组腺病毒载体能成功转染并表达于体外培养的皮肤组织块中；局部应用CTLA4Ig重组腺病毒载体使小鼠异体移植皮肤存活期延长至21d。结论用腺病毒载体局部转染CTLA4Ig能有效延长异体移植皮肤的存活。

Prolonging the survival of transplanted alloskin by transferring the CTLA4Ig locally with recombinant adenovirus vector in mice

Objective To find a simple and practical way to prolong the survival of transplanted alloskin to cover the burn and other wounds. Methods A recombinant CTLA_4Ig-Adenovirus expression vector was constructed by means of homorecombination, and then transferred either into cultured murine skin grafts ex vivo or local wound surface. The expression of CTLA_4Ig in cultured skin was immunohistochemically determined. Moreover, the survival time of transplanted alloskin from Kunming mice to Balb/c mice was evaluated. Results The CTLA_4Ig-Adenovirus vector could be successfully transferred and expressed in the cultured murine skin, and the locally used recombinant CTLA_4Ig-Adenovirus vector could significantlyprolong the survival of the transplanted alloskin from 8 d to 21 d. Conclusion Local transfection of the CTLA_4Ig-Adenovirus vector is a simple and effective way to prolong the survival of alloskin grafts.