Detection and genotyping of human papillomavirus in cervical samples from Italian patients |
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Authors: | De Francesco M A Gargiulo F Schreiber C Ciravolo G Salinaro F Manca N |
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Affiliation: | Institute of Microbiology and Virology, Spedali Civili-University of Brescia, 1 Piazzale Spedali Civili, 25123 Brescia, Italy. defrance@med.unibs.it |
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Abstract: | Human papillomaviruses (HPVs) are etiological agents of cervical cancer. In order to assess the epidemiological incidence and frequency of different HPV types, we applied a polymerase chain reaction (PCR)-direct sequencing approach based on the use of MY09/MY11 primers as compared to Hybrid Capture assay. Cervical samples were taken from 1,500 women, both with normal and abnormal cytological smears, and we found an incidence of 6.6% of HPV infection in Brescia. Overall, 97 samples tested HPV-positive, yielding 18 HPV types. The four most frequent HPV types were: HPV 16, -31, -6, and -58. This approach could be used in ordinary laboratory settings for quick and reliable typing of known and novel HPVs from clinical specimens and it could also be applied to anti-cancer vaccine development. |
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Keywords: | PCR HPV sequence |
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