抵抗素及其结合多肽对大鼠肝细胞糖原合成的影响

目的 探讨抵抗素(resistin)及其结合多肽(RBP)对大鼠肝细胞糖原合成的影响.方法 体外培养大鼠BRL肝细胞,分为空白对照组、抵抗素(60 ng/ml)干预组、RBP(1 nmol/L)干预组和抵抗素(60 ng/ml) RBP(1 nmol/L)联合干预组,培养2 h后筹集细胞,以蒽酮法检测胞内糖原含量,并采用RT-PCR法检测TRS2、SOCS3、GLUT2等基因mRNA的表达变化.结果 与空白对照组比较,抵抗素干预组糖原含量显著下降,但RBP干预组则无显著差异;与抵抗素干预组相比,抵抗素 RBP联合干预组糖原含量明显升高.各干预组BRL细胞中GLUT2基因的mRNA水平无显著差异.抵抗索干预组SOCS3 mRNA水平显著上调、IRS2 mRNA水平显著下凋;但两者在抵抗素 RBP联合下预组则尤明显变化.结论 RBP能拮抗抵抗素抑制肝细胞糖原合成的作用,但对正常肝细胞糖原合成功能无明显影响.

Effects of resistin and resistin binding peptide on glycogen synthesis in rat hepatocytes

Objective To investigate the effects of resistin and resistin binding peptide (RBP) on the glycogen synthesis of BRL hepatocytes in rats. Methods BRL cells were cultured in vitro and respectively treated with resistin (60 ng/ml,group R),RBP (1 nmol/ L,group RBP) ,resistin (60 ng/ml) plus RBP (1 nmol/ L) (group RR),or without intervention as the control (group C) for 2 hours. Glycogen content was detected. The expressions of glucose transporter 2 (GLUT2),IRS2 and SOCS3 gene mRNA were detected by RT-PCR. Resu...