| 肿瘤抑制因子Tob1对乳癌细胞的辐射增敏作用 |
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| 引用本文： | 焦旸,樊赛军,童建,曹建平,王国卿. 肿瘤抑制因子Tob1对乳癌细胞的辐射增敏作用[J]. 中国血液流变学杂志, 2007, 17(2): 202-204 |
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| 作者姓名： | 焦旸 樊赛军 童建 曹建平 王国卿 |
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| 作者单位： | 1. 苏州大学,放射医学与公共卫生学院,江苏苏州,215123
2. 苏州大学,医学院基础医学系,江苏苏州,215123 |
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| 摘 要： | 目的证明肿瘤抑制因子Tob1是否增加人类乳癌细胞系MDA-MB-231对电离辐射的敏感性,并初步探讨Tob1发挥辐射增敏作用的机制。方法采用集落形成实验探讨重组腺病毒介导的Tob1对MDA-MB-231辐射敏感性的影响,并通过Western Blot法检测由Tob1诱导的受γ线照射后乳癌细胞中相关蛋白表达水平的改变。结果重组腺病毒介导的Tob1高水平表达可显著增加乳癌细胞MDA-MB-231对电离辐射的敏感性(P<0.05),并可引起受照射后乳癌细胞中与凋亡及DNA损伤修复相关蛋白的表达水平改变。结论肿瘤抑制因子Tob1可能通过对细胞凋亡及DNA损伤修复相关蛋白的表达调控,增加乳癌细胞对电离辐射的敏感性。
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| 关 键 词： | 乳癌 辐射敏感性 |
| 文章编号： | 1009-881X（2007）02-0202-03 |
| 修稿时间： | 2007-03-30 |
The Tumor Suppressor Tob1 Sensitizes Breast Cancer Cells to Ionizing Radiation |
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| JIAO Yang, FAN Sai-jun, TONG Jian, et al. The Tumor Suppressor Tob1 Sensitizes Breast Cancer Cells to Ionizing Radiation[J]. Chinese Journal of Hemorheology, 2007, 17(2): 202-204 |
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| Authors: | JIAO Yang FAN Sai-jun TONG Jian et al |
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| Affiliation: | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China |
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| Abstract: | Objective To investigate the effects of the tumor suppressor Tob1 on radiosensitivity in a human breast cancer cell line MDA-MB-231.Methods Cell survival was determined by clonogenic assay by using adenovirus expression system(Ad5),and protein expression was analyzed by Western blot assay.Results It was demonstrated that pre-irradiation treatment with Ad5-Tob1 significantly increased the radiosensitivity of MDA-MB-231.Furthermore,Ad5-Tob1-mediated radiosensitivity was related to up-regulation of pro-apoptotic protein BAX and to down-regulation of several DNA DBS repair proteins.Conclusion Tob1 may be a radiosensitizer of breast cancer via increasing apoptosis and suppressing DNA repair. |
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| Keywords: | Tob1 |
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