通络方剂对糖尿病周围神经病大鼠模型的神经电生理及病理变化的影响

目的：研究通络方剂对糖尿病周围神经病（DPN）大鼠模型的神经生理功能及病理变化的影响，从而分析其治疗作用。方法：取35只SD大鼠，其中8只作为正常对照（NC）组，27只利用链脲佐菌素一次性腹腔注射诱导DPN大鼠模型，成功造模24只，再分为三组：小剂量通络方剂治疗（TL—CR1）组，大剂量通络方剂治疗（TLCR2）组和只造模不治疗的模型对照（DM）组，每组8只。对大、小剂量组分别予2g／kg和0．5g／kg通络方剂加2ml双蒸水配制成的溶液灌胃治疗8周，观察其对体重、血糖和尾神经运动、感觉神经传导速度（MCV、SCV）、潜伏期、波幅等神经电生理的变化，以及有髓纤维阳性面积、髓鞘面积、轴突面积等病理定量指标的影响。结果：两通络方剂治疗组与DM组的体重、血糖比较差异无统计学意义（P〉0．05）；运动潜伏期、MCV、运动与感觉神经动作电位波幅，两治疗组与NC组比较差异无统计学意义（P〉0．05），与DM组比较则有显著改善（P〈0．05）；两治疗组间比较差异无统计学意义（P〉0．05）；感觉潜伏期、SCV，大剂量组与NC组比较差异无统计学意义（P〉0．05），与DM组比较则有极显著改善（P〈0．001）；小剂量组与DM组比较有显著改善（P〈0．05），但又不及NC组和大剂量组（P〈0．05）。两治疗组有髓纤维阳性面积较DM组显著增加（P〈0．001），大剂量组较小剂量组更显著增加（P〈0．001）；小剂量组有髓纤维髓鞘面积较DM组显著增加（P〈0．01），大剂量组较DM组更显著增加（P〈0．001），且与小剂量组比较也显著增加（P〈0．05）；两治疗组有髓纤维轴突面积较DM组显著增加（P〈0．01），两治疗组间比较差异无统计学意义（P〉0．05）。结论：通络方剂对DPN大鼠模型的周围神经病变的电生理指标均有不同程度的改善，同时减轻了周围神经?

Effects of Tong-Luo composite recipe on electrophysiologic and pathologic changes in diabetic peripheral neuropathy rats

Objective： To investigate the effect of Tong-Luo composite recipe （TLCR）on electrophysiologic and pathologic changes in diabetic peripheral neuropathy（DPN） rats and analyse its therapeu tic effect. Methods： Healthy male Sprague Dawley rats were induced into animal models of DPN by a single abdominal injection of streptozotocin. The respective dosage of TLCR was given to the treated groups （low dosage group 0.5 g/kg and high dosage group 2 g/kg）hy stomach rearing once a day for 8 weeks. Then body weight, blood glucose and electrophysiologieal indexes such as motor conduction velocity （MCV）, sensory conduction velocity（SCV）, latency and amplitude of coccygeal nerve potential of each group were measured, and morphometric quantitative pathomorphometric analysis was also performed. Results： The body weight and blood glucose level of the TLCR groups were not significantly different from those of the diabetic model group （P〈 0.05）. The motor latency amplitude, and MCV and of the TLCR groups were not significantly different from those of the normal group（P〉0.05） , and they were significantly improved when compared with those of the model group（P〈0.05）. The indexes of the low dosage TLCR group were not significantly different from those of the high dosage TLCR group （P〉 0.05）. The sensory latency and SCV of the high dosage TLCR group were not significantly different from those of the normal group（P〉0.05） and they were significantly improved from those of the model group （P〈0. 001）. The indexes of the low dosage TLCR group were also significantly improved from those of the model group（P〈0.05） , but they were significantly different from those of the normal and high dosage TLCR groups（P〈0.05）. As compared with the model group,the TLCR treated groups had significantly larger myelinated nerve fiber positive areas（P〈0. 001）. Those of the high TLCR dosage group were larger than those of the low dosage TLCR group（P〈0. 001）. The myelin sheath ar