| 高温诱导颊癌细胞凋亡的相关机理研究 |
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| 引用本文： | 何永文,赵德萍,刘流,贾伟,谢春,李坚,黎明.高温诱导颊癌细胞凋亡的相关机理研究[J].口腔医学研究,2001,17(1):12-14. |
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| 作者姓名： | 何永文 赵德萍 刘流 贾伟 谢春 李坚 黎明 |
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| 作者单位： | 1. 昆明医学院第一附属医院
2. 云南省肿瘤研究所 |
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| 基金项目： | 云南省自然科学基金资助项目 !(编号 :1999C0 0 7R) |
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| 摘 要： | 目的 :探讨热诱导BcaCD885细胞凋亡的分子机理。方法 :水浴加温诱导BcaCD885产生凋亡。通过FCM DNA、FCM 抗体检测技术 ,观测热诱导颊癌细胞凋亡过程中bcl- 2及bax基因蛋白表达的动态变化。结果 :凋亡率与bcl- 2蛋白表达水平存在负相关关系 ,与bax蛋白表达水平存在正相关关系。结论 :高温作为一种外来刺激信号通过下调bcl 2基因的表达、上调bax基因的表达、上调bax基因的表达 ,诱导BcaCD885细胞凋亡的发生
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| 关 键 词： | 高温 BcaCD885细胞 凋亡 bcl2基因 bax基因 |
| 文章编号： | 1000—4661(2001)01—0012—03 |
| 修稿时间： | 2000年4月9日 |
The Mechanism of Apoptsis Induced by Hyperthermia in BcaCD885 Cells. |
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| HE Yong wen,ZHAO De ping,LIU Liu,et al..The Mechanism of Apoptsis Induced by Hyperthermia in BcaCD885 Cells.[J].Journal of Oral Science Research,2001,17(1):12-14. |
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| Authors: | HE Yong wen ZHAO De ping LIU Liu |
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| Institution: | HE Yong wen,ZHAO De ping,LIU Liu,et al.Department of Oral and Maxillofacial Surgery,1st Hospital,Kuming Medical College,Kunmin 650031 [ |
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| Abstract: | Objective:To study the molecular mechanism of apoptosis induced by hyperthemia in BcaCD885 cells.Methods:After inducing apoptosis through hyperthemia,we studied the apoptotic rate and the expression of bcl 2 and bax proteins with FCM DNA or antibody analysis technique.Results:After hyperthemia,there was down regulation of bcl 2 protein and strong induction of bax protein.The correlation coefficient r between the apoptotic rate and the expression of bcl 2 protein and bax protein was 0.8709 and 0.9240 respectively.Conclusion:Hyperthemia acting as an external signal may down regulate expression of bcl 2 gene or up regulate expression of bax gene and then induces apoptosis in BcaCD885 cells. |
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| Keywords: | Hyperthermia BcaCD885 cells Apoptosis bcl 2 gene bax gene |
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