羊膜移植修复兔结膜缺损的实验研究

目的：探讨羊膜移植修复结膜缺损的作用机制，为保存人羊膜移植的临床应用提供实验依据。方法：15只新西兰大白兔，取右眼作为羊膜移植组，左眼作为对照组，2组均建立兔结膜缺损动物模型，实验组进行羊膜移植，对照组不作移植，自然愈合。观察2组羊膜生长情况，并于术后3、5、7、9、14、21d取活体组织进行组织学检查。结果：羊膜移植组术后1周羊膜植片接近正常结膜，组织学检查证实羊膜与创伤处结缔组织紧密结合，结膜上皮沿缝合处爬行至羊膜表面，羊膜下仅有轻微的纤维增生，无瘢痕组织形成；对照组1周后创口开始愈合，组织学检查发现大量炎细胞浸润，纤维组织增生形成瘢痕。结论：保存人羊膜移植能有效地促进结膜上皮化的进行，减少瘢痕形成，最终减少并发症的发生。

An experimental study on amnion membrane transplantation for treatment of conjunctiva defect

Objective: To investigate the mechanism of amnion membrane transplantation(AMT) in treating conjunctiva defect.Methods: In fifteen New Zealand white rabbits,the right eyes were experimental group,the left eyes were control group,defect rabbit models were established,then AMT was performed on experimental group.Follow-up observation was carried out after surgery.Histopathological changes were examined at day 3,5,7,14,and 21.Results: Transplanted AM seemed to be normal conjunctiva in appearance.Histopathological examination showed that AM connected tightly with the defected tissue,conjunctiva epithelium migrated onto the AM surface,and there was slight fibrosis but no scar formation.Conclusions: AMT can effectively restore the epithelization of conjunctiva surface,inhibit scar formation and reduce complications after conjunctiva defect.