哌仑西平抑制豚鼠形觉剥夺性近视的视网膜机制

目的观察哌仑西平对豚鼠形觉剥夺性近视的抑制效果,探讨其可能的作用机制。方法 1周龄豚鼠60只随机分为4组,每组15只,分别为正常对照组(Ⅰ组)、单纯遮盖组(Ⅱ组)、哌仑西平组(Ⅲ组)与氯化钠组(Ⅳ组);各组右眼为观察眼,左眼为对照眼。6周后检测4组豚鼠双眼屈光度、眼轴长度,免疫组织化学法及Western blot检测视网膜多巴胺转运蛋白(dopamine transporter,DAT)的表达水平,并进行统计分析。结果Ⅱ,Ⅳ组观察眼相对屈光度及眼轴长度变化与Ⅰ组比较差异有统计学意义(P<0.05),Ⅲ组与Ⅰ组比较差异无统计学意义(P>0.05);视网膜DAT阳性细胞数和DAT蛋白表达Ⅱ,Ⅳ组明显低于Ⅰ,Ⅲ组(P<0.05),Ⅰ组与Ⅲ组,Ⅱ组与Ⅳ组比较差异均无统计学意义(P>0.05)。结论哌仑西平能抑制豚鼠形觉剥夺性近视的发展,阻止近视视网膜DAT表达水平下降,推测哌仑西平可能通过影响视网膜多巴胺系统而抑制近视的发展。

Mechanism of pirenzepine on form deprivation myopia in guinea pigs

Objective To observe the effect of pirenzepine on inhibiting form deprivation myopia(FDM) in guinea pigs and to study its mechanism.Methods A total of 60 one-week-old guinea pigs were randomly divided into 4 groups: group Ⅰ(normal controls),group Ⅱ(form-deprivation),group Ⅲ(pirenzepine solution),and group Ⅳ(sodium chloride).FDM was established in the right eyes of each guinea pig.The left eyes served as control eyes.Ocular refraction and axial length were measured 6 weeks later.Immunohistochemistry and Western blot were used to analyze the expression of retinal dopamine transporter(DAT).These data were statistically analyzed.Results There were significant differences in refractive error and axial length between group Ⅰ and Ⅱ,and between group Ⅰ and Ⅳ(P<0.05),and there were no significant differences between group Ⅰ and Ⅲ(P>0.05).The count of DAT positive cells and DAT protein level were lower in group Ⅱ and Ⅳ than those in group Ⅰ and Ⅲ(P<0.05).There were no significant differences between group Ⅰ and Ⅲ,and between group Ⅱ and Ⅳ(P>0.05).Conclusion Pirenzepine can prevent the development of FDM,and inhibit the decrease of DAT expression.It is indicated that pirenzepine may block the development of FDM by influencing dopamine transporter system.