大鼠佐剂性关节炎模型表现特征及评价指标

目的:描述大鼠佐剂性关节炎(Adjuvant-induced arthritis,AA)临床表现主要指标的评价方法。方法:完全弗氏佐剂免疫SD大鼠诱导AA模型,进行全身及关节炎指数评分、计算关节肿胀数、测定足爪肿胀度、进行足爪X线摄片、踝关节病理检查及评分。结果:大鼠免疫后11天(d11)出现足爪红肿,全身及关节炎指数评分和关节肿胀数增加。炎症高峰期在免疫后d19～d28。大鼠出现活动障碍,可见耳朵、鼻和尾根部"关节炎"结节。X线可见关节软组织肿胀、骨密度降低、骨侵蚀病灶以及关节间隙狭窄甚至消失。踝关节病理见滑膜组织增生,炎细胞浸润,血管翳出现等。结论:大鼠AA是兼有局部关节炎症和全身表现的类风湿性关节炎动物模型。关节炎指数、关节肿胀数、足爪肿胀度、足爪X线摄片、踝关节病理等是评价AA模型临床表现的主要指标。

Clinical manifestations and the main evaluation method on adjuvant-induced arthritis model in rats

Objective:To describe clinical manifestations and the main evaluation method on adjuvant-induced arthritis model in rats.Methods:The rat AA model was induced by a single intradermal injection of CFA into the rat footpad.Arthritis was evaluated by global assessment,arthritis index,the number of swelling joints,paws swelling.X-ray radiography was taken on the paws.Pathohistology of ankles was observed and scored.Results:Day 11 after immunization,the paws of AA rats appeared red and swelling,both the scores of global assessment and the number of swelling joints were enhanced.Day 19-28 after immunization,the inflammation reach its peak.The behaviors of rats were obstructed,and arthritic nodes were observed on ears,noses and tails.X-ray of paws showed that the soft tissues were swelling,the density of bone was reduced,bone erosion was observed,and the joint space was narrow or vanished.Pathohistology of ankles joints showed that the synovium of AA rats were hyperplasia,cartilage was destroyed,pannus was formed,and inflammatory cells infiltrated into synovium.Conclusion:Rat AA model is an animal model for rheumatoid arthritis which perform local joint inflammation and system symptom.Arthritis index,the number of swelling joints,paws swelling,X-ray and pathohistology of ankles and so on are the principal evaluation method on AA model.