GnRH在宫颈鳞状细胞癌中的表达及意义

目的:从蛋白质及核酸水平研究GnRH(促性腺激素释放激素)在宫颈鳞状细胞癌中的表达,以探讨其存在的临床意义。方法:采用免疫组化和原位杂交两种实验方法,分别从蛋白质和核酸水平检测45例宫颈鳞状细胞癌及20例慢性宫颈炎中GnRH的表达。结果:在20例慢性宫颈炎宫颈上皮组织中GnRH及GnRH mRNA表达均为阴性,而在45例宫颈鳞状细胞癌组织中,GnRH阳性表达率为80%,GnRH mRNA阳性表达率为64.44%,即无论是在蛋白质水平还是在核酸水平比较两者均显示差异有显著性;定性及定量比较GnRH及GnRH mRNA在不同临床期别的宫颈癌组织中的表达情况,结果为GnRH定性表达率Ⅰ期50%(6/12),Ⅱa期85.7%(18/21),Ⅱb-期100%(12/12);GnRH mRNA定性表达率,Ⅰb期33.3%(4/12),Ⅱa期71.4%(15/21),Ⅱb-期83.3%(10/12);GnRH及GnRH mRNA在定性上表达有显著差别(P<0.05)。结论:宫颈鳞状细胞癌组织存在较高的GnRH,其有可能参与宫颈鳞状细胞癌发生、发展,为进一步研究通过抑制GnRH的产生,减少宫颈癌的发生及治疗宫颈癌提供了一定的理论基础。

Expression of gonadotropin releasing hormone(GnRH) in cervical squamous cell carcinoma and its significance

Objective:To study GnRH expression in cervical squamous cell carcinoma to explore its probable clinical significance.Method:GnRH in cervical squamous cell carcinoma and chronic cervicitis were detected by two respective methods of immunohistochemistry and in situ hybridization.Results:The expression of GnRH and GnRH mRNA in epithelium of 20 chronic cervicitis cases were negative but positive in 45 cervical squamous cell carcinoma cases.Positive rate was 80% for GnRH and 64.44% for GnRH mRNA.The expression of both protein and nucleic acid levels had statistically significant difference between chronic cervicitis and cervical squamous cell carcinoma.Qualitative and quantitative comparison for GnRH and GnRH mRNA were conducted between different clinical stages of cervical carcinoma tissues,the quantitative expression rate of GnRH were Stage Ⅰ 50%(6/12),StageⅡa 85.7%(18/21),StageⅡb-100%(12/12);and the quantitative expression rate of GnRH mRNA were StageⅠb 33.3%(4/12),StageⅡa 71.4%(15/21),StageⅡb-83.3%(10/12);qualitative comparison revealed significant differences(P<0.05).The different expression of GnRH and GnRH mRNA in different clinical stage of cervical cancer showed qualitatively significant differences(P< 0.05).Conclusions: GnRH and GnRH mRNA could be detected in cervical squamous cell carcinoma and might be involved in the development process.Our study provides a theoretical basis for a hypothesis that inhibition of GnRH secretion could help reducing the incidence and also a new treatment for cervical squamous cell cancer.