| HPLC—ELSD法测定排石胶囊中熊果酸的含量 |
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| 引用本文： | 徐富柱,程龙. HPLC—ELSD法测定排石胶囊中熊果酸的含量[J]. 中国民族民间医药杂志, 2012, 21(5): 19-20 |
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| 作者姓名： | 徐富柱 程龙 |
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| 作者单位： | 徐富柱 (江苏省南京同仁堂药业有限责任公司,江苏南京,210012) ； 程龙 (江苏省南京同仁堂药业有限责任公司,江苏南京,210012) ； |
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| 摘 要： | 目的：测定排石胶囊中熊果酸的含量。方法：采用HPLC—ELSD法。色谱柱为Lichrospher5-C18柱，流动相为甲醇一水一冰醋酸（83：17：0．1），流速为1．0mL·min-1，进样量为20uL，柱温为35℃，ELSD检测器漂移管温度为72℃，载气流量为1．9L·min-1。结果：熊果酸进样量在6．4μg-22．4μg范围内与峰面积常用对数的积分值线性关系良好（r=0．9991），加样回收率为98．7％，RSD=2．1％。结论：本方法操作简便、准确、灵敏度高、重复性好，可作为排石胶囊的质量控制方法。
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| 关 键 词： | 排石胶囊 HPLC—ELSD 熊果酸 |
Content Determination of Ursolic Acid in Paishi Capsules by HPLC - ELSD |
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| Xu Fu - zhu,Cheng long. Content Determination of Ursolic Acid in Paishi Capsules by HPLC - ELSD[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2012, 21(5): 19-20 |
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| Authors: | Xu Fu - zhu Cheng long |
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| Affiliation: | Nanjing Tongrentang Pharmaceutical CO. LTD. Nanjing 210012, China |
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| Abstract: | Objective： To establish the method for the content determination of Ursolic Acid in Paishi Capsules. Methods： HPLC - ELSD method was adopted. The determination was performed on Agilent C18 column with mobile phase consisted of methgl al- cohol -water- acetic acid （83：17： 0. 1 ） at the flow rate of 1.0 ml min-l. The injection volume was 20 ul and column temperature was 35 ℃. The drift tube temperature was 72℃ and the flow rate of carrier gas was 1.9L/min - 1. Results： The linear range of Ursol- ic Acid was 6. 4μg -22.4μg （r =0. 9991 ） . Average recoveries was 98. 7%, RSD =2. 1%. Conclusion： The method is simple and accurate, and has good sensitivity and reproducibility. It could be used for quality control of Paishi Capsules. |
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| Keywords: | Paishi Capsules HPLC - ELSD Ursolic Acid |
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