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| 耐异质性利奈唑胺金黄色葡萄球菌筛选及耐药机制分析 | |
| 引用本文： | 王敬华,虞培娟,沈懿,王守立,童年,朱雪明. 耐异质性利奈唑胺金黄色葡萄球菌筛选及耐药机制分析[J]. 中华医院感染学杂志, 2011, 0(17): 3535-3538 |
| 作者姓名： | 王敬华 虞培娟 沈懿 王守立 童年 朱雪明 |
| 作者单位： | 1. 苏州大学附属第二医院检验科,江苏苏州,215004 2. 张家港市红十字血站,江苏张家港,215600 3. 苏州大学基础医学系,江苏苏州,215004 4. 辉瑞制药有限公司,江苏苏州,215006 |
| 基金项目： | 江苏省自然科学基金，苏州市卫生局科技兴卫青年专项基金 |
| 摘 要： | 目的建立耐异质性利奈唑胺葡萄球菌筛选方法,并对其耐药机制进行初步分析。方法常规方法检测葡萄球菌属临床分离株对万古霉素、替考拉宁、头孢西丁、利奈唑胺耐药性,采用菌谱分析法筛选耐异质性利奈唑胺葡萄球菌,琼脂稀释法和E-test法检测利奈唑胺MIC值,PCR方法检测其耐药cfr基因。结果未检出利奈唑胺中介或耐药菌株;耐异质性利奈唑胺金黄色葡萄球菌的检出率为1.04%;琼脂稀释法和E-test法检测异质性耐药菌株MIC结果一致;未检出利奈唑胺耐药cfr基因。结论利奈唑胺对葡萄球菌属在体外具有较强抗菌活性,该研究未检出利奈唑胺中介或耐药葡萄球菌属,其耐药机制和临床意义有待进一步研究。 |
| 关 键 词： | 异质性利奈唑胺 耐药性 金黄色葡萄球菌 |
Detection of heterogeneous linezolid-resistant Staphylococcus aureus and study on linezolid-resistance mechanism |
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| WANG Jing-hua,YU Pei-juan,SHEN Yi,WANG Shou-li,TONG Nian,ZHU Xue-ming. Detection of heterogeneous linezolid-resistant Staphylococcus aureus and study on linezolid-resistance mechanism[J]. Chinese Journal of Nosocomiology, 2011, 0(17): 3535-3538 | |
| Authors: | WANG Jing-hua YU Pei-juan SHEN Yi WANG Shou-li TONG Nian ZHU Xue-ming |
| Affiliation: | WANG Jing-hua,YU Pei-juan,SHEN Yi,WANG Shou-li,TONG Nian,ZHU Xue-ming(The Second Affiliated Hospital of Suzhou University,Suzhou,Jiangsu 215004,China) |
| Abstract: | OBJECTIVE To develop a method for detection the heterogeneous of linezolid-resistant Staphylococcus aureus(hLRSA),and to study its antibiotic resistance mechanism.METHODS Population analysis was used to detect hLRSA from clinical isolates of Staphylococci,agar dilution method and E-test were used to detect MICs,and PCR was applied to scan cfr gene from hLRSA.RESULTS No linezolid-intermediated or drug resistant strains were detected The detection rate of hLRSA(out of 192 strains) was 1.04%.Agar dilution meth... |
| Keywords: | Heterogeneous linezolid Drug resistance Staphylococcus aureus |
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