小鼠Lewis肺癌细胞与胚胎干细胞体外共培养的生物学行为☆

背景：从发生学角度来看，肿瘤细胞和胚胎干细胞都受原癌基因调控，并具有惊人的分裂能力。将胚胎癌细胞移植入正常发育的同系小鼠的胚泡内，结果产生了不具有恶性表型的嵌合体小鼠，表明在胚胎环境中肿瘤细胞的命运可以发生改变。目的：探讨小鼠胚胎干细胞对Lewis肺癌细胞生物学行为变化的影响。方法：通过Transwell小室体外共培养小鼠胚胎干细胞与Lewis肺癌细胞。实验组Transwell小室中为成纤维细胞和胚胎干细胞，12孔板中为肿瘤细胞；对照组将加入到Transwell小室中的胚胎干细胞替换为成纤维细胞，保持小室中细胞的总数不变，12孔板中为肿瘤细胞。结果与结论：与对照组比较，实验组Lewis肺癌细胞形态变化显著，部分细胞出现老化甚至凋亡迹象；实验组Lewis肺癌细胞的增殖明显减慢(P < 0.05)，穿透DB胶生物膜的Lewis肺癌细胞数明显减少(P < 0.05)。提示胚胎干细胞能够抑制Lewis肺癌细胞的生长增殖及侵袭力。

Biological behavior of mouse Lewis lung cancer cells co-cultured with embryonic stem cells in vitro

BACKGROUND: From a genetic perspective, the tumor cells and embryonic stem cells (ESCs) are regulated by the proto-oncogene and have amazing ability to divide. Embryonic cancer cells were transplanted into blastocysts of normal development mice and produced chimeric mice without the malignant phenotype. The fate of tumor cells can change with embryonic existence. OBJECTIVE: To study the effect of mouse ESCs on the changes in biological behaviors of Lewis lung cancer cells. METHODS: Mouse ESCs were cocultured with Lewis lung cancer cells using Transwell chamber in vitro. In the experimental group, fibroblasts and ESCs were added in the Transwell chamber, whereas tumor cells were in the 12-well plate. In the control group, fibroblasts and fibrocytes were added in the Transwell chamber. The total number of cells were in the chamber did not change, and tumor cells were in the 12-well plate. RESULTS AND CONCLUSION: Compared with the control group, the experimental group shows significant difference in morphology of Lewis lung cancer cells; some cells appear signs of aging and apoptosis. The proliferation of Lewis lung cancer cells was significantly decreased (P < 0.05), and the number of Lewis lung cancer cells that could traverse DB gel biomembrane was significantly reduced in the experimental group (P < 0.05). Results indicate that ESCs can inhibit the growth, proliferation and invasiveness of Lewis lung cancer cells.