Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae |
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| Authors: | Fooladi AA Imani Islamieh D Iman Doust R Hosseini A Karami SM Marashi |
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| Affiliation: | 1. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;2. Department of Microbiology, Faculty of Advance Sciences and Technology, Islamic Azad University-Pharmaceutical Sciences Branch, Tehran, Iran;3. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;4. Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran |
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| Abstract: | ObjectiveTo study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.MethodsThree important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.ResultsAccording to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.ConclusionsResult show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran. |
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