系统性红斑狼疮患者血清IgG对骨髓造血干/祖细胞抑制和凋亡的研究

目的：观察系统性红斑狼疮（SLE）患者血清IgG类抗体体外造血抑制活性及其与造血细胞过度凋亡的关系.方法：应用甲基纤维素集落培养法、原位末端标记及流式细胞术观察系统性红斑狼疮患者血清IgG在体外抑制正常骨髓造血干/祖细胞的增殖及促进正常CD34＋细胞的凋亡.结果：活动期SLE患者血清IgG体外可明显抑制正常骨髓粒-巨噬系祖细胞（CFU-GM）和红系祖细胞（CFU-E）的增殖.这种IgG可特异吸附于正常CD34＋细胞表面,加速CD34＋细胞凋亡,上调CD34＋细胞Fas抗原表达水平.结论：活动期SLE患者血清IgG在体外可抑制正常骨髓造血干/祖细胞的增殖.机制与其上调CD34＋ Fas抗原表达水平而促进CD34＋细胞凋亡有关.

The hematopoietic inhibitor of serum IgG from patients with systemic lupus erythematosus and relationship with the apoptosis of CD34+ cells

Objective:To explore the hematopoietic inhibitor of serum IgG from patients with systemic lupus erythematosus and relationship with the apoptosis of CD34~+ cells.Methods:The inhibiting activity to the growth of CFU-E and CFU-GM of normal bone marrow was analysed by in vitro methycelluose culture with normal or SLE serum IgG and CD34~+ cell apoptosis was examined with a kit of programmed cell death assay.Results:The serum IgG of active SLE patients suppressed significantly the colony formation of both CFU-GM and CFU-E of normal bone marrow than inactive SLE,control groups(P<0.01).The active patients IgG could bind specifically to normal CD34 hematopoietic cells and induce CD34~+ cells apoptosis,as well as increase the expression of Fas antigen on CD34~+ cells surface.Conclusion:Fas-mediated induction of CD34~+ cells apoptosis by serum IgG from active patients with systemic lupus erythematosus may cause inhibition of hematopoiesis.