| 7种单纯疱疹病毒Ⅱ型IgG抗体检测试剂盒的比较 |
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| 引用本文： | 余艳华,尹跃平,袁柳凤,朱邦勇,孙秀峰. 7种单纯疱疹病毒Ⅱ型IgG抗体检测试剂盒的比较[J]. 中国艾滋病性病, 2010, 0(2) |
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| 作者姓名： | 余艳华 尹跃平 袁柳凤 朱邦勇 孙秀峰 |
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| 作者单位： | 中国医学科学院北京协和医学院皮肤病研究所中国疾病预防控制中心性病控制中心;广西皮肤病防治研究所; |
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| 基金项目： | 卫生部全国性病防控工作项目~~ |
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| 摘 要： | 目的比较7种单纯疱疹病毒Ⅱ型(HSV-2)特异性IgG抗体检测试剂盒的优劣。方法对来自体检和性病门诊的生殖器疱疹可疑者的189份血清,同时用3种进口试剂盒(A、B、C)和4种国产试剂盒(D、E、F、G)进行检测。结果以"扩大金标准"为判断标准,7种检测试剂盒的敏感性:A、B、C分别为94.6%、100%、100%,D、E、F、G分别为98.5%、93.9%、96.9%、30.8%;特异性:A～G分别为98.2%、96.4%、96.4%、9.1%、43.6%、18.2%、100%;受试者工作特性曲线(ROC)下面积:A～G分别为0.964(0.933～0.995)、0.982(0.953～1.011)、0.982(0.953～1.011)、0.529(0.434～0.623)、0.664(0.567～0.761)、0.517(0.416～0.618)、0.654(0.576～0.732)。结论三种进口试剂盒检测结果可信度高,可用于临床实验室的辅助诊断、流行病学调查等。
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| 关 键 词： | 单纯疱疹病毒Ⅱ型 IgG抗体 酶联免疫吸附法 |
Comparison of seven ELISA kits for detecting anti-HSV2 specific IgG antibodies |
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| YU Yan-hua,YIN Yue-ping,YUAN Liu-feng,et al.. Comparison of seven ELISA kits for detecting anti-HSV2 specific IgG antibodies[J]. Chinese JOurnal of Aids & STD, 2010, 0(2) |
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| Authors: | YU Yan-hua YIN Yue-ping YUAN Liu-feng et al. |
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| Affiliation: | YU Yan-hua,YIN Yue-ping,YUAN Liu-feng,et al. ( Institute of Dermatology,Chinese Academy of Medical Sciences & Peking Union Medical College,Reference laboratory,National Center for STD control,China CDC,Nanjing,Jiangsu 210042,China) |
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| Abstract: | Objective To compare the validity of seven ELISA kits for detecting anti-HSV2 specific IgG antibodies. Methods A total of 189 serum specimens were collected from suspected HSV2 cases, and detected by three imported and four homemade ELISA kits for anti-HSV2 specific IgG antibodies. Results Compared with the expanded golden standard, the sensitivity and specificity of these ELISA kits were 94.6% and 98.2%, 100% and 96.4%, 100% and 96.4%, 98.5% and 9.1%, 93.9% and 43.6%, 96.9% and 18.2%, 30.8% and 100%, respe... |
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| Keywords: | Herpes simplex virus type II (HSV2) IgG antibody Enzyme-linked immunosorbent assay(ELISA) |
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