神经生长因子对大鼠脑血肿周围细胞凋亡的影响

目的：观察脑出血后血肿周围神经细胞凋亡和Caspase-3的表达，以及NGF（神经生长因子）对上述情况的影响。方法：自体血注入法制备大鼠脑出血模型，经TUNEL染色和Caspase-3免疫组化染色，照片观察并计数阳性细胞数，结果用SPSS 11．5软件统计分析。结果：假手术组无TUNEL细胞表达，Caspase-3少量表达；脑出血组6h血肿周边组织出现TUNEL阳性细胞，72h达高峰，Caspase-3在脑出血后6h表达开始增高，24h达到高峰，脑出血后血肿周围TUNEL阳性细胞与Caspase-3的表达呈正相关（r=0．371，P〈0．05）；应用NGF干预后，自12h起各时间点TUNEL细胞和Caspase-3表达均较脑出血组减少（P〈0．05），以各自高峰时间最为显著（p〈0．05），但未能改变TUNEL细胞和Caspase-3表达的高峰时间。结论：（1）脑出血后血肿周围细胞凋亡与Caspase-3表达有关。（2）NGF能够有效地抑制脑出血后的神经细胞凋亡。

The effect of nerve growth factor on cell apoptosis of intracerebral perihematoma in rats

Objective:To observe the effect of the nerve growth factor(NGF) on cell apoptosis and caspase-3 expression on rats intracerebral hematoma.Method: The intracerbral hematoma rat model was made by autologous blood injection.TUNEL dyeing and caspase-3 immunochemical technique were used to observe the cell change after cerebral hemorrhage edema.Results were analyzed statistically using software of SPSS11.5.Results: There is no TUNEL positive cell expression in sham operation group,and only a few caspase-3 positive cells expressed.While in intracerebral hemorrhage group,TUNEL positive cells expressed around perihematomal brain at 6 h and peaked at 72 h after hemorrhage;Caspase-3 positive cells expressed also at 6 h but peaked at 24 h after hemorrhage.There offered a direct correlation between the expression of TUNEL and caspase-3(r=0.371,P<0.05).They were decreased from 12 h after hemorrhage by intervened with the injection of NGF(P<0.05),decreased significantly at their own peak time(P<0.05)but their peak time did not change.Conclusions:(1) The apoptosis around perihematomal brain after intracerebral hemorrhage is related to the expression of caspase-3.(2) The apoptosis after intracerebral hemorrhage can be inhibited efficiently by using the injection of NGF,and it offers a very bright perspective in treatment of intracerebral hemorrhage.