首页 | 本学科首页   官方微博 | 高级检索  
     


Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells
Authors:Leticia Boldrin MESTIERI  Ana Lívia GOMES-CORNéLIO  Elisandra Márcia RODRIGUES  Loise Pedrosa SALLES  Roberta BOSSO-MARTELO  Juliane Maria GUERREIRO-TANOMARU  Mário TANOMARU  FILHO
Affiliation:1.- Universidade Estadual Paulista, Escola de Odontologia, Departamento de Odontologia Restauradora, Araraquara, SP, Brazil.;2.- Universidade de Brasília, Instituto de Biologia, Departamento de Biologia Celular, Brasília, DF, Brazil.
Abstract:Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus.

Objective

The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs).

Material and Methods

The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%).

Results

MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.

Conclusions

The hDPCs were suitable for the evaluation of new endodontic materials in vitro. MTAP may be considered a promising material for endodontic treatments.
Keywords:Dental pulp   Silicate cement   Cell viability   Alkaline phosphatase
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号