| 竹节参总皂苷对人白血病细胞株HL-60的作用 |
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| 引用本文： | 袁丁,左锐,张长城. 竹节参总皂苷对人白血病细胞株HL-60的作用[J]. 中西医结合学报, 2007, 5(5): 570-572 |
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| 作者姓名： | 袁丁 左锐 张长城 |
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| 作者单位： | 1. 三峡大学医学院,湖北,宜昌,443002;三峡大学天然产物研究与利用湖北省重点实验室,湖北,宜昌,443002
2. 三峡大学医学院,湖北,宜昌,443002 |
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| 基金项目： | 湖北省教育厅科研项目;湖北省卫生厅科研项目 |
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| 摘 要： | 目的:研究竹节参总皂苷(total saponins of Panax japonicus,TSPJ)对人白血病细胞HL-60的作用,探讨其作用机制,为进一步开发竹节参提供理论依据。方法:体外培养人早幼粒白血病细胞HL-60,细胞计数法观察TSPJ对细胞活力的影响,四唑氮蓝(nitroblue tetrazolium,NBT)测定其对细胞还原能力的影响,流式细胞仪检测细胞周期变化和膜表面分化抗原CD11b的表达情况。结果:和阴性对照组比较,不同浓度TSPJ处理的细胞活力显著下降,NBT阳性细胞增多,G_0/G_1期细胞增多,S期细胞减少,膜表面分化抗原CD11b的表达量增多。结论:TSPJ对体外培养的HL-60细胞生长育一定抑制作用,其作用机制与诱导细胞分化和周期阻滞有关。
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| 关 键 词： | 竹节参 HL-60细胞 细胞分化 |
| 文章编号： | 1672-1977（2007）05-0570-03 |
Effects of total saponins of Panax japonicus on human leukemic HL-60 cells |
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| Ding YUAN,Rui ZUO,Chang-cheng ZHANG. Effects of total saponins of Panax japonicus on human leukemic HL-60 cells[J]. Journal of Chinese integrative medicine, 2007, 5(5): 570-572 |
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| Authors: | Ding YUAN Rui ZUO Chang-cheng ZHANG |
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| Affiliation: | 1. Medical College, Three Gorges University, Yichang, Hubei Province 443002, China; 2. Hubei Province Key Laboratory of Natural Products Research and Development, Three Gorges University, Yichang, Hubei Province 443002, China |
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| Abstract: | Objective:To study the effects of total saponins of Panax japonicus(TSPJ)on human leukemic HL-60 cells. Methods:Human leukemic HL-60 cells were cultured in vitro.The cancer cell vigor was detected by using cell counting kit-8.Nitroblue tetrazolium(NBT)was used for measuring cell reduction.The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry. Results:Compared with the negative control group,TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression,while the numbers of NBT positive cells and cells in G_0/G_1 phase in the different concentrations of TSPJ-treated groups were increased. Conclusion:TSPJ can inhibit the HL-60 cell growth in vitro.Its mechanism may be related to inhibiting prolif- eration and inducing cell differentiation and cycle arrest. |
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| Keywords: | Panax japonicus HL-60 cells cell differentiation |
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