严重创伤及合并内毒素攻击后小鼠肝、肺组织巨噬细胞CD14和清道夫受体的表达及其意义

目的　进一步揭示创伤及合并内毒素攻击后 ,小鼠肝、肺组织内CD14和清道夫受体(scavengerreceptor,SR)的表达变化规律及器官间的差异性。　方法　小鼠 84只分为正常对照组 (4只 )、创伤组 (40只 )、创伤合并内毒素攻击组 (40只 )。采用双侧股骨骨折合并内毒素血症小鼠模型 ,以免疫组化方法检测肝、肺组织巨噬细胞CD14和SR在创伤后 2 ,4 ,6 ,9,13h时的动态变化。　结果　肝组织巨噬细胞CD14和SR分别在创伤后 9h发生上调和下调 (P <0 .0 5 ) ,肺组织巨噬细胞CD14和SR分别在创伤后 6h发生上调和下调 (P <0 .0 5 )。创伤合并内毒素攻击后 ,肝、肺组织巨噬细胞CD14和SR均于伤后 2h分别呈显著的上调和下调改变 (P <0 .0 5和 0 .0 1) ,并呈显著负相关关系 (r=- 0 .82 3,- 0 .797,P <0 .0 1) ,而且创伤后 6 ,9,13h时肺组织内CD14和创伤后 4hSR表达变化幅度大于肝组织 (P <0 .0 5和 0 .0 1)。　结论　创伤及合并内毒素攻击后 ,肝、肺组织内CD14和SR表达发生双向调节 ,CD14表达上调和SR表达下调可能与炎症反应由“自控”向“失控”转化有关。肝、肺组织内CD14和SR表达上的差异可能与创伤及合并内毒素攻击时器官功能损害的序贯性相关。

Expressions of CD14 and scavenger receptor on macrophages of liver and lung after severe trauma and endotoxin challenge in mice

Objective To further study the dynamic expressions of CD14 and scavenger receptor(SR)on the macrophages of the liver and the lung and their organic differences in mice. Methods Experimental mice were randomly divided into three groups including control group (4 mice), trauma group (Group TM, 40 mice) and traumatic endotoxin challenge group (Group TM+ET, 40 mice). The model mice with both femoral fracture and endotoxemia were used. The expressions of the CD14 and the SR on the macrophages of the liver and the lung were detected at the post traumatic 2nd, 4th, 6th, 9th and 13th hour in immunocytochemistry. Results After trauma, the expressions of the CD14 and the SR on the macrophages of the liver showed the tendency of up and down regulation respectively 9 hours after trauma ( P <0.05), while that of the lung showed the tendency of up and down regulation respectively 6 hours after trauma ( P <0.05). Furthermore, the post traumatic endotoxin challenge induces up regulation of the CD14 and down regulation of the SR significantly 2 hours after trauma ( P <0.05 and 0.01). Correlation analysis indicated that the CD14 and the SR had significantly negative relationship in both the liver and the lung ( r =-0.823,-0.797, P <0.01); meanwhile, there exist some differences between the liver and the lung on kinetics of the CD14 and the SR ( P <0.05 and <0.01). Conclusions The dynamic expressions of the CD14 and the SR on macrophages of the liver and the lung is characterized by two side modulation after trauma and endotoxin challenge, which may be a symbol of inflammatory transformation from self control to out of control. The expression differences of the CD14 and the SR in the liver and the lung may be correlated with the sequential organic injury after severe trauma and endotoxin challenge.