GENOMIC STRUCTURE OF MOUSE TBXZ AND DETECTION OF EXPRESSION OF TBXZ IN NORMAL AND MALIGNANCE MELANOPHORE BY RT-PCR

Objective: Sequencing of mouse Tbx2 gene andobserving the expression of Tbx2 gene in normal andmalignant melanophore. Methods: The PCR productsof TbX2 cDNA were cloned into PUC18 vector andsequenced. The normal and malignant melanocytes wereused to extract total RNA. The expression of Tbx2 genewas detected by RT-PCR. Results: The TbXZ genome iscomposed of seven e-cons and six nitrons. No expressionof Tbx2 gene in the normal melanocytes was noted, butall malignant melanocytes showed expression of TbXZgene. Conclusion: The observation showed the analysisof the genomic structure of mouse TbX2. TbX2 plays acritical role during the development of the malignantmelanophore.