| 肝炎诊断芯片60-mer长链寡核苷酸探针的设计研究 |
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| 引用本文： | 孙朝晖,马文丽,危敏,王蜀燕,郑文岭.肝炎诊断芯片60-mer长链寡核苷酸探针的设计研究[J].军医进修学院学报,2007,28(1):63-65. |
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| 作者姓名： | 孙朝晖 马文丽 危敏 王蜀燕 郑文岭 |
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| 作者单位： | 1. 广州军区广州总医院,检验科,广州,510010
2. 南方医科大学基因工程研究所,广州,510515 |
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| 基金项目： | 广东省广州市科技攻关项目 |
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| 摘 要： | 目的:通过生物信息学软件对易引起慢性肝炎的三种肝炎病毒基因组结构进行分析,设计肝炎病毒诊断分型芯片的寡核苷酸(O ligo)探针。方法:利用BLAST软件对乙型肝炎病毒(HBV)、丁型肝炎病毒(HDV)、丙型肝炎病毒(HCV)6个亚型的DNA及cDNA序列进行序列比对,得到有意义的特异序列;然后用生物信息学软件Array designer 3.0设计特异性高、Tm值相近、长度均一的O ligo探针。结果:获得156条60-m er探针,用于打印成DNA芯片,进行HBV、HDV的联合检测和HCV的基因分型。结论:利用BLAST检索系统和生物学软件Array desig-ner 3.0设计肝炎病毒诊断分型芯片的探针,是一种简便可行、有效的方法。
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| 关 键 词： | 肝炎病毒 乙型 肝炎病毒 丁型 肝炎病毒 丙型 基因芯片 寡核苷酸探针 计算生物学 软件 |
| 文章编号： | 1005-1139（2007）01-0063-03 |
| 收稿时间： | 2006-03-06 |
| 修稿时间： | 2006-07-18 |
Design of 60 mer oligonucleotide probes for microarray detection and genotype of hepatitis virus |
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| SUN Zhao-hui,MA Wen-li,WEI Min,WANG Shu-yan,ZHENG Wen-ling.Design of 60 mer oligonucleotide probes for microarray detection and genotype of hepatitis virus[J].Academic Journal of Pla Postgraduate Medical School,2007,28(1):63-65. |
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| Authors: | SUN Zhao-hui MA Wen-li WEI Min WANG Shu-yan ZHENG Wen-ling |
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| Institution: | Clinical Laboratory, General hospital of guangzhou command Guangzhou 510010, China |
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| Abstract: | Objective:To design oligonucleotide probes for microarray detection and genotype of hepatitis virus.Methods:By analyzing the DNA or cDNA of HBV,HDV and HCV of 6 different genotypes with BLAST program,a group of specific sequences for the candidate probes was specified.Array designer 3.0 software was applied to analyze the candidates to select the probes with high specificity,identical length and similar melting temperature(Tm).Results:Altogether 156 oligonucleotide probes were designed,and deposited on oligonucleotide chips as the microarray for hepatitis virus detection and genotype.Conclusion:BLAST and Array designer 3.0 software are effective for designing the oligonucleotide probes. |
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| Keywords: | hepatitis B virus hepatitis C virus hepatitis D virus microarray oligonucleotide probes computational biology software |
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