阿魏酸钠对糖尿病大鼠肾脏糖基化终产物受体mRNA表达的影响

 目的探讨阿魏酸钠(sodium ferulate,SF)对糖尿病(diabetes mellitus,DM)大鼠肾脏皮质糖基化终产物受体(receptor for advanced glcation end products,RAGE)mRNA表达的影响。方法 对链脲佐菌素(streptozotocin,STZ)诱导的DM大鼠灌胃给予SF110 mg·kg^-1·d^-1),治疗8周,测定各组大鼠肾重／体重、血尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,Scr)、24h尿蛋白定量,并用RT-PCR方法检测肾脏皮质RAGE mRNA的表达,观察肾脏病理改变。结果 DM组大鼠肾重／体重,BUN,Scr,24h尿蛋白定量,肾皮质RAGE mRNA的表达显著高于正常对照组;SF组肾重／体重,BUN,24 h尿蛋白定量,肾皮质RAGE mRNA的表达显著低于DM组;DM组大鼠肾脏病理显著异常,SF可显著减轻其病理学改变。结论SF可通过抑制肾脏RAGE mRNA的表达,减轻糖基化终产物(advanced glycation end products,AGEs)-RAGE之间的相互作用对DM大鼠肾脏产生保护作用。

Effects of sodium ferulate on the mRNA of receptor for advanced glycation end products in kidneys of diabetic rats

OBJECTIVE To investigate the effects of sodium ferulate (SF)on the mRNA of receptor for advanced glycation end products (RAGE) in kidneys of diabetic rats. METHODS The diabetic rats induced by streptozotocin (STZ) were treated with SF (110 mg·kg^-1·d^-1 for 8 weeks. The renal weight/body weight, blood urea nitrogen (BUN), serum creatinine (Scr) and proteinuria was measured. The expression of RAGE mRNA in renal cortex was measured with RT-PCR. The pathological changes were also observed. RESULTS The levels of BUN, Scr, proteinuria, the expression of RAGE mRNA in renal cortex, and renal weight/body weight in diabetic control group were significantly higher than that in normal group. The levels of BUN, proteinuria, the expression of RAGE mRNA in renal cortex, and renal weight/ body weight in the group of treatment were significantly lower than that in diabetic control group. The pathological changes were significant in the diabetic control group, which were significantly ameliorated with the treatment of SF. CONCLUSIONS SF can inhibit the expression of RAGE mRNA in kidneys and reduce the mutual action between RAGE and AGEs, which maybe the mechanisms of the protecting effects of SF treatment on kidneys in diabetic rats.