广西壮、汉族绝经后妇女维生素D 受体基因型与骨密度、骨代谢的关系

目的探讨维生素D受体基因(VDR)型在壮、汉族绝经后妇女中的分布及其与骨密度、骨代谢的关系.方法在广西居住20年以上的绝经后汉族妇女116名,壮族妇女82名.记录年龄、绝经年龄,测量身高、体重.采用双能X线吸收法测定骨密度(BMD);用聚合酶链反应-限制性片段长度多态性(PCR -RFLP)法测定受试者的VDR基因型;测定血清骨钙素(osteocalcin,OC)、尿脱氧吡啶啉(deoxypyridinoline,DPD)和尿肌酐(creatinine,Cr).结果壮、汉族妇女年龄、绝经年限、体重、体重指数、BMD、VDR基因型频率分布无显著性差异(P>0.05);BB、Bb、bb基因型检出率分别为6.57%、66.16%和27.27%;BB基因型组第2腰椎(L2)BMD比bb基因型组低10.03%,第4腰椎(L4)BMD分别较bb、Bb基因型组低9.63%和12.44%(P<0.05);BB基因型组骨质疏松发生率最高(46.15%),Bb基因型组次之(19.85%),bb基因型组最低(14.81%)(P<0.05);BB基因型组OC最低,与Bb、bb 基因型组比较也有显著性差异(P<0.05);三组间尿DPD排泄率(DPD/Cr)差异无统计学意义.结论 VDR基因型可作为预测广西壮、汉族绝经后妇女骨质疏松危险性的遗传学标志.

Relationship between vitamin D receptor genotypes and bone mineral density, bone metabolism in Guangxi Chuang and Han national postmenopausal women

Objective To explore the distribution of vitamin D receptor (VDR) genotypes and the association of VDR genotypes with bone mineral density (BMD) and bone metabolism in Guangxi Chuang and Han national postmenopausal women. Methods 116 Han and 82 Chuang national postmenopausal women were included in this study. Their age, age at natural menopause were recorded, height, weight were measured, VDR genotypes were determined by PCR-RFLP and bone mineral density was measured by double energy X absorption (DEXA). Their serum osteocalcin (OC), urinary deoxypyridinoline (DPD) and creatinine (Cr) excretion also were measured. Results Age, BMD and the distribution of the VDR genotypes didn' t differ significantly by nationality (P>0.05). The tested rate of BB, Bb and bb genotype was 6.57%, 66.16% and 27.27%, respectively. The BMD of L_2 in BB subgroup was 10.03% lower than that in bb subgroup, the BMD of L_4 was 9.63 and 12.44% lower than those in bb subgroup and Bb subgroup, respectively (P<0.05). The osteoporosis morbidity in BB subgroup was higher than those in Bb and bb subgroups (P<0.05). Conclusions VDR genotypes could be the genetics predictor for osteoporosis of postmenopausal women.