| 玻璃化深低温冷冻保存气管的实验研究 |
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| 引用本文： | 徐洪,史宏灿,卢丹,吴俊.玻璃化深低温冷冻保存气管的实验研究[J].生物医学工程学杂志,2008,25(5). |
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| 作者姓名： | 徐洪 史宏灿 卢丹 吴俊 |
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| 作者单位： | 1. 扬州大学,临床医学院,胸心外科,扬州225001
2. 扬州大学,临床医学院,妇产科,扬州,225001 |
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| 基金项目： | 国家自然科学基金资助项目(30672080);;江苏省卫生厅医学重大科研课题资助(K200410);;江苏省高校自然科学研究计划项目资助(04KJB320163) |
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| 摘 要： | 玻璃化深低温冷冻在降温过程中大大减少冰晶的形成，极大的减轻了冷冻保存过程中对组织、细胞的损伤，前景广阔。本文旨在研究玻璃化快速降温对气管组织结构的影响，实验分为玻璃化快速降温组、程控缓慢降温组、新鲜未冷冻组3组，通过HE染色、TUNEL染色、透射电镜及扫描电镜等检查，评价不同冷冻方法对气管软骨和纤毛结构影响。结果显示，玻璃化快速降温组的气管软骨板结构完整，黏膜上皮及其纤毛结构、软骨细胞形态保持良好，与传统程控缓慢降温相比，快速降温冷冻对软骨细胞损伤轻，有更高的软骨细胞存活率和纤毛上皮的覆盖率，获得较高的软骨复苏率，较好地保持形态结构完整，其保存效果优于传统缓慢降温的保存效果。
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| 关 键 词： | 气管 玻璃化 深低温冷冻 冷冻保护剂 |
Experimental Study of Cryopreserving Trachea by Vitrification |
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| Xu Hong,Shi Hongcan,Lu Dan,Wu Jun.Experimental Study of Cryopreserving Trachea by Vitrification[J].Journal of Biomedical Engineering,2008,25(5). |
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| Authors: | Xu Hong Shi Hongcan Lu Dan Wu Jun |
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| Institution: | Department of Cardiothoracic Surgery;College of Clinical Medicine;Yangzhou University;Yangzhou 225001;China;Department of Gynaecology and Obstetrics;China |
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| Abstract: | Vitrification is a promising alternative to tissue preservation, which will greatly avoid the ice-crystal formation and circumvent the hazardous effects associated with ice formation during the entire procedures. In this study, we evaluate the effect of vitreous cryopreservation of rabbit trachea by comparing the vitrification procedure with the conventional computer-programmed slow freezing approachses. Harvested tracheae were tailored and divided into groups and cryopreserved by vitrification and programm... |
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