Two monoclonal antibodies detecting allotypic determinants of HLA-A |
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| Authors: | S. MINAMI R. UEDA T. SHIRAKI M. TANIMOTO R. NAMIKAWA M. SETO K. NISHIDA I. TSUGE K. OTA T. TAKAHASHI |
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| Affiliation: | First Department of Internal Medicine, Japan;Laboratory of Chemotherapy, Japan;Aichi Red Cross Blood Center, Nagoya, Japan;First Department of Pathology, Nagoya, Japan;Experimental Pathology, Nagoya, Japan;Department of Pediatrics, Nagoya University School of Medicine, Nagoya, Japan;Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan |
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| Abstract: | Three mouse hybridomas producing cytotoxic antibodies against HLA were established. By standard microcytotoxicity test against panels of normal controls, the antigen defined by MA-9 antibody (IgM) showed a good correlation with HLA-A9 alloantigen detected by conventional typing alloantisera (r = 1.0). Family studies also showed that MA-9 determinant segregated with HLA-A9. MA-10 antibody (IgM) reacted with all HLA-A10 positive lymphocyte donors and cross-reacted with two thirds of HLA-AW33 positive donors. M1-1 antibody (IgG2a) reacted with all the panel cells tested and immunoprecipitated a molecule of 43,000 daltons from Nonidet P-40 lysates of 3H-glucosamine-labelled cells. The results showed that MA-9 and MA-10 antibodies can be used as routine tissue typing reagents. |
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