运用辣根过氧化物酶和乙酰胆碱酯酶双标记法对家兔腰、骶、尾髓脊髓小脑束乙酰胆碱酯酶阳性神经元的研究

本研究采用10只家兔进行腰、骶、尾髓的脊髓小脑束乙酰胆碱酯酶阳性神经元定位研究。其中8只在小脑内注射HRP后,按Mesulam组化法追踪腰、骶、尾髓各节段的HRP-AChE双标及HRP单标神经元;作为对照,2只家兔同样节段的脊髓切片则单纯显示神经元的AChE活性。实验结果表明,家兔腰、骶、尾髓的各群脊髓小脑束神经元中,大多数细胞均为乙酰胆碱酯酶阳性神经元,但它们所含AChE的量可能有所不同。各群细胞中乙酰胆碱酯酶阳性神经元所占数量的多少可能与脊髓小脑束神经元的不同功能有关。

THE CHOLINESTERASE-CONTAINiNG NEURONS OF THE SPINO-CEREBELLAR TRACTS IN THE LUMBO-SACRO-COCCYGEAL CORD OF THE RABBIT. AN EXPERIMENTAL STUDY BY USING HRP-AChE DOUBLE LABELING TECHNIQUE

10 rabbits were used in the present study for localization of the cholinergic neurons of the spinocerebellar tracts in the lumbo-sacro-coccygeal cord. In 8 cases of them, the HRP-AChE double labeled and HRP single-labelcd neurons in these segments of the spinal cord were tarced with Mesulam's histochemical technique. The sections from the same segments of the other 2 cases were only stained histochemically for the AChE activity. The result of the present study suggests that the majority of the spinocerebellar neurons in the lumbo-sacro-coccygeal cord of the rabbit should be cholinergic, although the amounts of AChE in those neurons may be different. The population of the cholinergic neurons in dif-ferent cell groups of the spinocerebeliar tracts might be related with the functional differences between them.