氧化型低密度脂蛋白诱导血管内皮细胞基因表达谱的改变

通过对氧化型低密度脂蛋白诱导血管内皮细胞基因表达谱改变的研究，为阐明氧化型低密度脂蛋白致内皮细胞功能障碍及动脉粥样硬化形成的分子机制提供科学依据。采用含有4000条全长已知人类基因cDNA以及96条参照基因cDNA克隆制作的基因表达谱芯片，筛查氧化型低密度脂蛋白（100mg/L)作为24h对人脐静脉血管内皮细胞的基因表达谱改变的影响。结果显示，氧化型低密度脂蛋白可诱导1条基因表达下调（泛肽激活酶），3条基因表达上调（血清和糖皮质激素诱导的蛋白激酶、热休克蛋白70和KDRF）。结果发现，在氧化型低密度脂蛋白作用的早期阶段可诱导内皮细胞相关基因的表达改变；首次发现氧化型低密度脂蛋白可诱导内皮细胞KDRF、泛肽激活酶和血清和糖皮质激素诱导的蛋白激酶基因表达改变，为揭示氧化型低密度脂蛋白引起血管内皮细胞功能障碍的机制提供了研究成果。

Changes of the Gene Expression Pattern of Vascular Endothelial Cells Induced by Oxidized Low Density Lipoprotein

Aim To study the change of the gene expression pattern of vascular endothelial cells induced by oxidized low density lipoprotein. Methods After human umbilical vein endothelial cells was exposed to oxidized low density lipoprotein(100 mg/L) for 24 h, genes expression was screened with a complementary DNA microarray representing 4 000 different human genes and 96 control genes. Results There were 1 down regulated gene and 3 up regulated genes. The down regulated gene was ubiquitin activating enzyme E1. The up regulate genes were as follows: serum and glucocorticoid inducible protein kinase (SGK), heat shock protein 70 (HSP70), and KM 102 derived reductase like factor (KDRF). Conclusions Oxidized low density lipoprotein could induce the change of gene expression pattern of endothelial cells. It was the first time to find that oxidized low density lipoprotein could induce the change of the gene expression of SGK, HSP70 and KDRF. All the changed genes were related to the stress stimulation. These changes might be related to the dysfunction of endothelial cells induced by oxidized low density lipoprotein.