The functional half-life of H-2Kd-restricted T cell epitopes on living cells |
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Authors: | Gé rard Eberl,Christian Widmann,Giampietro Corradin |
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Abstract: | The functional half-life (t1/2) of different complexes formed by major histocompatibility complex (MHC) class I molecules and antigen on the surface of target cells was measured using specific cytotoxic T lymphocyte (CTL) clones in cytolysis, and interferon-γ-production and Ca2+-mobilization assays. Functional t1/2 values of 5–10 h were obtained, which are in accordance with some previous estimations obtained from biochemical and immunochemical measurements. Moreover, these values were independent of the type of target cell, fixation of the target cells, or proteases able to degrade the peptides, suggesting that the unfolding of the peptide/MHC complexes at the cell surface alone determines the functional t1/2 of the CTL epitopes. |
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Keywords: | T lymphocyte Cytotoxicity Peptide Major histocompatibility complex |
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