Heat shock increases antigenic peptide generation but decreases antigen presentation |
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Authors: | Elsa Pé pin,Christian L. Villiers,Franç ise M. Gabert,Vincent A. Serra,Patrice N. Marche,Maurice G. Colomb |
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Abstract: | The heat shock response is a universal and highly conserved cellular response to stress. We describe here the effect of elevated temperature on the capacity of B cells to present antigen. Heat shock markedly affects the ability of these cells to process and present tetanus toxin to class II-restricted T cell clones. Inhibition of antigen presentation is due neither to a modification of antigen capture nor to a variation of major histocompatibility complex (MHC) class II molecule synthesis and cell surface expression. Stressed and nonstressed B cells are able to present peptides loaded at the cell surface with the same efficiency. Nevertheless, heat shock leads to an increase of antigen peptide generation in subcellular compartments; an enhancement of cathepsin B activity is also observed. These data suggest that such a stress induces a failure in the intracellular peptide loading onto MHC class II molecules. |
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Keywords: | Antigen presentation Heat stress Major histocompatibility complex class II molecule Tetanus toxin Heat shock protein |
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