不同时间深冷冻异体骨移植的免疫原性研究

异体骨的冷冻处理方法与其移植后修复骨缺损的临床效果密切相关。本研究将实验控制在不同冷冻时间的条件下，对异体皮质骨进行深冷冻处理（－１９６℃），采用ＤＢＡ／２和Ｃ＿（５７）ＢＬ／６两组主要组织相容性集合体（ＭＨＣ）完全不相同时近交系小鼠作为动物模型，观察１０分钟、２０分钟、２周、４周、四个不同时间深冷冻异体骨移植的免疫原性，发现经１０分钟和２０分钟的深冷冻处理在一定程度上可以降低异体骨的免疫原性，其抗体－补体介导的细胞杀伤反应与异体新鲜骨相比有显著性差异（Ｐ＜０．０１）。然而经２周、４周的深冷冻处理可使异体骨的免疫原性更进一步降低，其抗体一补体介导的细胞杀伤反应与同基因新鲜骨相比无显著性差异（Ｐ＞０．０１）。本实验结果为临床处理和应用深冷冻异体骨提供了依据。

THE IMMUNOGENICITY OF DEEP-FROZEN ALLOGENIC BONE TREATED FOR VARIOUS TIMES AFTER TRANSPLANTATION

AbstractImmunigenical Departement of Fourth Mililtory University Two inbred strains of mode adult mise(C_(67)BL/6,DBA/2)were used as animal,modelwith different major histocomptibility(MHC)loci.The immunogenicity of corticalbone allografts which were deep-frozen in a liquid nitrogen freezer(-196 degree,centigra-de)for 10 minute,20 minute,2 weeks and 4 weeks were evaluated in vitro using antibody-complement mediated cytotoxcity(ACMC)assay. The 10 minute and 20 minute deep-freezing treatment resulted in a moderate decreasing the immunogenicity of allogenic bone,Whereas the 2 weeks and 4 weeks deep-freezing treatment resulted in a significantly reducedimmunogenicity of allogenic bone and show no significant difference from fresh isogenicconrtical bone(P>0.01)in ACMC assay .