抗核抗体荧光模型特异性抗体谱及滴度与自身免疫性疾病的关联性研究

目的：探讨抗核抗体（ ANA）荧光模型、滴度及特异性抗体谱与自身免疫性疾病的关联性。方法对404例自身免疫性疾病（ AID）患者，同时采用间接免疫荧光法检测ANA和欧蒙印迹法检测15项特异性抗核抗体谱（ ANAS），对比分析ANA荧光模型和滴度以及ANA荧光模型在不同疾病中的表达，并与健康体检者300名进行比较。结果在AID组中的ANA检出阳性率为68.81％，滴度在1∶1000以上者占75.90％。检出的ANA荧光模型，以胞浆型和核均质型为主，分别检出130例和108例。 ANA阳性患者滴度升高，检出荧光混合模型的比例也明显增加。结论 AID患者中ANA检出率较高，高滴度的ANA阳性患者荧光模型以混合模型为主，同时检测ANA和ANAS对自身免疫性疾病诊断具有重要的临床意义。

Study on the relationship between antinuclear antibody fluorescence model,specific antibodies spectrum,ti-ter and autoimmune diseases

Objective To investigate the relationship between antinuclear antibody （ ANA ） fluorescence model, specific antibodies spectrum, titer and autoimmune diseases（AID）.Methods Four hundred and four pa-tients with AID were investigated by using indirect immunofluorescence assay to detect ANA, at the same time, using Europe and Mongolia blotting to detect 15 specific antinuclear antibodies spectrum （ ANAS）, and a comparative analy-sis was performed of expression of ANA fluorescence model and the titer and ANA fluorescence model in different dis -eases.Results In AID group the positive rate of ANA was 68.81%, titers in 1∶1 000 accounted for 75.90%.ANA fluorescence model mainly showed cytoplasmic type and nuclear homogeneous type, 130 cases and 108 cases respec-tively.In ANA positive patients, the titer of ANA was higher and fluorescent hybrid model was also increased. Con-clusion The detection rate of ANA was higher in patients with AID. In fluorescent ANA positive patients with high titer the fluorescence model mainly is hybrid model. The simultaneous detection of ANA and ANAS has important clinical significance for diagnosis of autoimmune diseases.