Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum |
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| Authors: | Liang Guo, Jianhui Xie, Yuanyuan Ruan, Lei Zhou, Haiyan Zhu, Xiaojing Yun, Yan Jiang, Long Lü , Kangli Chen, Zhihui Min, Yumei Wen,Jianxin Gu, |
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| Affiliation: | 1. Gene Research Center, Shanghai Medical College and Institutes of Biomedical Science of Fudan University, Shanghai, 200032, PR China;2. Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, PR China;3. Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, PR China;4. Key Laboratory of Medical Molecular Virology, Institute of Medical Microbiology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China;1. Department of Medicine and Molecular Science, Hiroshima University, Japan;2. Department of Endoscopy, Hiroshima University, Japan;1. Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Campus Virchow Clinic, Charité-University Medicine, Berlin, Germany;2. Institute of Edible fungi, Shanghai Academy of Agricultural Sciences, PR China;1. Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China;2. School of Pharmaceutics Science, Jiangnan University, Wuxi 214122, PR China;1. Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, PR China;2. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, PR China;3. Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR China |
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| Abstract: | Spores of Ganoderma lucidum contain a large amount of bioactive substances and have a higher bioactivity than the fruit bodies of G. lucidum. However, ingredients from spores are less studied due to the difficulties in collecting the spores and breaking the rigid shell. In this study, a water-soluble polysaccharide named GSG was extracted from the spores of G. lucidum. GSG is characterized to be a branched glucan that contains several different kinds of linkages. It was an effective inducer of MAPKs- and Syk-dependent TNF-α and IL-6 secretion in murine resident peritoneal macrophages. Dectin-1 could recognize GSG and partially mediate its biological activities. Additionally, in vivo administration of GSG potentiated the Con A-induced proliferative response of splenocytes and induced anti-tumor activity against Lewis lung cancer in mice. Therefore, these results suggest that GSG is an effective immunomodulator and may be a promising adjuvant remedy for anti-tumor therapies. |
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| Keywords: | Anti-tumor Glucan Dectin-1 Ganoderma lucidum Spores |
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