血糖对大鼠轻、重两型急性胰腺炎的识别作用探讨

目的探讨血糖(BS)对大鼠急性胰腺炎(AP)轻重的识别作用,为临床应用提供动物试验依据。方法24只Wistar大鼠随机均分为3组:假手术组(SO)、轻型急性胰腺炎组(MAP)和重症急性胰腺炎组(SAP)。每组均在术后1 h、7 h和12 h抽血查血淀粉酶(AMY)、血糖(BS),并于术后 12 h杀死大鼠,取大鼠心、肺、肝、肾、胰行病理检查,比较三组血AMY、BS变化规律和重要脏器损伤数目。结果(1)MAP组BS在7 h、12 h时间点比2 h明显降低(P<0．05)。(2)SAP组BS在7 h、12 h时间点比2 h明显增高(P<0．01)。(3)SAP组BS与SO、MAP组的比较,明显增高(P<0．01)。 (4)SO、MAP组大鼠无重要脏器损伤,而SAP组合并有重要脏器损伤,尤其以肺和肝较多见。结论 AP程度越重血糖增高幅度越大。

The clinic value of blood sugar on distisuish mild and severe acute pancreatitis in wistar rats

Objective To study the significance of blood sugar(BS) to distinguish mild acute pancreatitis with severe acute pancreatitis. Methods 24 healthy Wistar rats were randomly divided into 3 groups: sham operative group (SO), mild acute pancreatitis group(MAP) and severe acute pancreatitis group(SAP), each group was divided into 2 h,7 h,12 h sub-group again. After models were induced, we tested the serum concentration of amylase(AMY), blood sugar and observed the pathologic result of heart, lung, liver, kidney and pancreas. Results (1) BS had apparently decreased in MAP 7 h,12 h sub-group compared to 2 h sub-group(P<0.05). (2) Compared to SO or MAP group, BS increased significantly in SAP group(P<0. 01). (3) Wistar rats had no important organ injury in SO, MAP groups, but had some important organs injury in SAP group. Conclusion It could distinguish MAP with SAP to test BS.