肺炎患者支气管肺泡洗液中激肽形成机制的探讨

目的：探讨下呼吸道急性炎症时激肽生成途径。方法：测定肺炎患者（肺炎组）和健康志愿者（对照组）支气管肺泡灌洗液（ＢＡＬＦ）中激肽，激肽形成酶活性，血浆血管舒缓素（ＰＫ）和α２－巨球蛋白（α２－Ｍ），并鉴定激肽形成酶活性分子来源。结果：肺炎组ＢＡＬＦ中的各种检测指标均明显高于对照组（Ｐ〈０．００１）；经凝胶过滤和酶抑制试验鉴定，肺炎患者ＢＡＬＦ中主要激肽形成酶活性位于高分子量部分，ＰＫ抑制物能显著抑制

Kinins generation pathway in bronchoalveolar lavage fluid from patients with pneumonia

Objective: To investigate the kinins generation pathway in acute airway inflammation Methods: The bronchoalveolar lavage fluid (BALF) from patients with pneumonia and healthy individuals was collected to determine kinins, plasma kallikrein, a-2 macroglobulin and kinins generation enzyme activity Results: Kinins and kinins generation activity in the BALF were significantly higher in patients with pneumonia than in the controls (P<0 001) Gel filtration and enzyme activity inhibition test showed that the kinins generation activity in BALF from patients with pneumonia was inhibited by plasma kallikrein Conclusion: Kinins and kinins generation activity in acute airway inflammation are significantly increased and kinins seems to be mainly generated by plasma kallikrein