鲍曼不动杆菌基因同源性研究

目的分析鲍曼不动杆菌的基因同源性，并对其进行分子分型。方法采用重复序列聚合酶链反应技术（rep—PCR），分别以基因外重复回文序列（REP）和肠杆菌基因间重复一致序列（ERIC）为引物，对122株鲍曼不动杆菌基因组DNA进行扩增并分型。结果REP—PCR将鲍曼不动杆菌分为14种基因型（A～N）。其中H型含98株，为主要的流行型别，并可分为6个亚型；ERIC—PCR将菌株分为13种基因型（A’～M’），其中L’型100株，为主要的流行型别，并可分为5个亚型。其余菌株在其他基因型中均为零星分布。结论在鲍曼不动杆菌基因同源性分析中，REP—PCR的分辨率较ERIC—PCR高；同时表明我院存在着某一基因型鲍曼不动杆菌的感染流行，估计该型菌株可能以克隆株的形式播散。

Analysis of gene homology in Acinetobacter baumannii isolates

Objective To investigate the gene homology of Acinetobacter baumannii in our hospital and their typing.Methods The genomic DNA of 122 strains were analyzed by repetitive element polymerase chain reaction(rep-PCR) using repetitive extragenic palindromic(REP) and enterobacterial repetitive intergenic consensus sequence(ERICS). Results There were 14 genotypes(A to N) in all isolates with REP-PCR mainly including type H of 98 strains classified to 6 subtypes and 13 genotypes(A' to M') with ERIC-PCR mainly including type L' of 100 strains classified to 5 subtypes,respectively.The distribution of remaining strains were sporadic in the other genotypes. Conclusion The power of REP-PCR is higher than that of ERIC-PCR in discriminating typing of the Acinetobacter baumannii.In addition,in our hospital there was the prevalence of a certain genotype of Acinetobacter baumannii,and it can be clonal dissemination.