缺氧对肺腺癌细胞生长特性及血管形成的影响

目的探讨缺氧对肺腺癌肿瘤生长及血管形成的影响。方法人肺腺癌细胞株A549暴露于常氧(空气，5%CO2)、缺氧(1％O2，5％CO2，94%N2)、无氧(95％N2，5%CO2)环境48h后，将细胞接种于裸鼠皮下，观察其生长情况。通过免疫组化染色计数微血管密度，测定移植瘤组织中血管内皮细胞生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)的表达水平。结果移植10天后，缺氧组肿瘤体积显著大于常氧组。移植25天后缺氧组肿瘤体积、重量、微血管密度以及瘤组织中VEGF、bFGF水平均显著高于常氧组，而无氧组显著低于常氧组。结论适度缺氧可以刺激肺癌细胞VEGF、bFGF等生长因子的表达，促进移植瘤血管形成，提高肿瘤的生长能力；而严重缺氧损伤癌细胞，影响生长因子合成表达，阻碍血管形成，抑制肿瘤的生长。

Effect of hypoxia on growth and angiogenesis of non-small cell lung cancer

Objective To realize the effect of hypoxia on the growth and angiogenesis of non small cell lung carcinoma. Methods Non small cell lung carcinoma cell (A549) were exposed to hypoxia(1%O 2,5%CO 2,90%N 2) or anoxia(95%N 2,5%CO 2) condition for 48 hours. The cells exposed to hypoxia were subcutaneously planted in nude mice, observed the volume per 5days. After 25 days, the weight and volume were measured, and the microvessel density (MLD) were observed by immunohistochemistry method for CD34, the level of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were assayed by ELISA. Results Comparing with normoxia group, the volume and weight of subcutaneous tumor of nude mice were increased in hypoxic group, the MLD and the level of VEGF and bFGF in hypoxic group were higher than that in normoxia group. The volume, weighty, MLD and the level of VEGF and bFGF in anoxic groups decreased contrasted to normoxic group. Conclusion Moderate hypoxia can up regulate the expression of VEGF and bFGF, and increase the angiogenesis and growth of non small cell lung carcinoma. But serious hypoxia can decrease the expression of VEGF and bFGF, and inhibit the angiogenesis and growth of the carcinoma.