低氧致鼠腺泡内肺动脉PDGF—B链蛋白表达的变化

目的：探讨血小板源性生长因子（ＰＤＧＦ）在低氧肺动脉高压血管构形重建发生中的作用。方法：应用免疫组化技术结合计算机图像分析，检测了低氧大鼠腺泡内肺动脉（ＩＡＰＡ）ＰＤＧＦ－Ｂ链蛋白表达水平。结果：常氧时，ＩＡＰＡ仅有ＰＤＧＦ－Ｂ链蛋白弱表达；低氧１天时，ＩＡＰＡ便有较强的ＰＤＧＦ－Ｂ链蛋白表达，定位于ＩＡＰＡ的内皮和中膜，低氧３天至１４天仅分布于中膜；低氧１、３、５、７、１４天各时间点ＰＤＧＦ－Ｂ链蛋白表达分别为常氧组的１．５３、１．５９、１．５６、１．６２和１．４２倍，差异有显著性（Ｐ＜０．０１）。结论：ＰＤＧＦ－Ｂ链蛋白可能参与了低氧肺动脉高压血管构形重建的发病过程

Hypoxia induced the expression of platelet derived growth factor (PDGF) B chain protein by intra acinar pulmonary arteries

Purpose To study the role for PDGF in mechanisms for vascular remodeling of hypoxic pulmonary hypertension. Methods Immunohistochemical technique combined with computer image analysis was used to determine expression of PDGF B chain protein by intra acinar pulmonary arteries (IAPA) in rat exposed to hypoxia. Results A weak PDGF B chain protein expression was observed in IAPA of rat in normoxia. However, a stronger PDGF B chain protein expression was found in endothelium and medium of IAPA in hypoxia for 1 day and only in medium of IAPA from day 3 to 14. Computer image analysis indicated that compared with normoxic group, the hypoxic group showed the 1 5,1 56,1 62, and 1 42 fold increase in the PDGF B chain protein content of IAPA, for 1,3,5,7 and 14 days, respectively ( P <0.01). Conclusion The increased PDGF B chain protein expression may participate in the process of vascular remodeling in pulmonary hypertension caused by hypoxia.