地塞米松和环孢菌素A对IL-4、IL-5合成的抑制作用

为研究淋巴细胞在哮喘发病中的作用及地塞米松（Ｄｅｘ）、环孢菌素Ａ（ＣｓＡ）治疗哮喘的机理，用ＥＬＩＳＡ法检测哮喘患者和对照组的外周血单个核细胞（ＰＢＭＣ）在植物血凝素刺激下合成白细胞介素４（ＩＬ－４）、白细胞介素５（ＩＬ－５）水平，以及Ｄｅｘ和ＣｓＡ对哮喘患者的ＰＢＭＣ合成ＩＬ－４、ＩＬ－５的抑制作用。结果表明，哮喘组的ＩＬ－４、ＩＬ－５水平较对照组高（Ｐ＜０．０５）；１０－６ｍｏｌ／Ｌ的Ｄｅｘ和ＣｓＡ均能抑制哮喘组ＰＢＭＣ合成ＩＬ－４、ＩＬ－５，增大药物浓度至２×１０－６、３×１０－６ｍｏｌ／Ｌ时，ＣｓＡ对ＩＬ－５合成的抑制作用逐步增强；Ｄｅｘ对ＩＬ－５合成的抑制作用较ＣｓＡ强（Ｐ＜０．０５）。提示糖皮质激素和ＣｓＡ抑制ＩＬ－４、ＩＬ－５的合成是其治疗哮喘的机理之一。

INHIBITORY EFFECT OF DEXAMETHASONE AND CYCLOSPORIN AON IL4,IL5 PRODUCTS

To study the effects of lymphocyte in the mechanism of asthma and the mechanism of dexamethasone(Dex) and cyclosporin A (CsA) in treating asthma, ELISA was used to measure IL4 and IL5 concentration in peripheral blood mononuclear cells (PBMC) from asthmatic patients and health control, and the inhibitory effect of Dex and CsA on IL4,IL5 products of PBMC from asthmatic patients. PBMC were stimulated by phytohaemagglulinin(100mg/L). The results showed IL4 and IL5 products in asthmatic patients were higher than that in health control (P<0.05). Increasing drug concentration to 210-6,310-6mol/L,IL5 product were farther inhibited by CsA (P<0.05). The inhibitory effect of Dex to IL5 product was stronger than that of CsA. (P<0.05). The results indicated that the inhibition of IL4 and IL5 products may be one of mechanisms of glucocorticoid and CsA to treat asthma. It is likely that the effect of Dex in treating asthma is more effective than CsA.