胃癌组织中CTGF蛋白表达与细胞凋亡的相关性

目的探讨结缔组织生长因子（connective tissue growth factor,CTGF）蛋白在胃癌组织中的表达及它与细胞凋亡间的关系。方法应用免疫组织化学方法检测CTGF在10例正常胃粘膜、50例癌旁组织和胃癌细胞中的表达,同时利用原位末端标记技术（TUNEL）检测癌组织中的细胞凋亡指数（AI）,并分析它们之间的关系。结果 CTGF正常组织的阳性表达率为10%,在癌旁组织的表达率为30%,在胃癌组织的表达率为32%;凋亡指数在CTGF阴性组为5.72±1.12,在CTGF阳性组为1.32±1.08,两组比较差异有显著性（P〈0.001）。CTGF的阳性表达与胃癌的分化程度、临床分级、淋巴转移与否有关（P〈0.05）。但与胃癌患者的年龄、性别、肿瘤直径、浸润深度无明显关系（P〉0.05）。结论 CTGF可能在胃癌凋亡过程中起重要作用。

RELATIONSHIP BETWEEN EXPRESSIONS OF CTGF PROTEIN WITH APOPTOSIS IN GASTRIC CARCINOMA

Objective To detect the expression of connective tissue growth factor（CTGF）in gastric carcinoma and the relationship with apoptosis.Methods The expression of CTGF in 10 cases of normal gastric mucosa and 50 cases of gastric carcinoma were detected by immunohistochemistry.At the same time,apoptosis index（AI） was detected the in cancer tissues using TUNEL technology.Results The expression of CTGF was higher in gastric carcinoma（32%） than normal gastric mucosa（10%）,（P=0.02）.The AI between the CTGF positive group was（1.32±1.08） and control group was 5.72±1.12,P 0.001.Expression of CTGF was highly correlated with the carcinoma＇s degree of differentiation and expression of CTGF in lymph node metastasis（P0.05）.There was no obvious relationship among CTGF and tumors site,invasion depth and the patients age and sex（P 0.05）.Conclusion CTGF may play an important role in the gastric cancer apoptosis process.