| 融合毒素TOP_3表达载体的构建 |
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| 引用本文： | 刘芳,党国全. 融合毒素TOP_3表达载体的构建[J]. 青海医学院学报, 2010, 31(3): 154-156,165 |
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| 作者姓名： | 刘芳 党国全 |
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| 作者单位： | 青海大学医学院生物化学与分子生物学教研室 |
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| 摘 要： | 目的构建融合毒素TOP3的原核表达载体,为研究该融合毒素的功能及分析评价临床应用前景奠定基础。方法构建原核表达载体pET-28a（＋）-TAT-ODD-CASPASE3[pET-28a（＋）-TOP3],并在大肠杆菌BL21（DE3）pLysS内诱导表达。结果经酶切分析及DNA序列分析,所构建的质粒均插入TAT-ODD-CASPASE3融合基因。结论成功构建原核表达载体pET-28a（＋）-TOP3,并在IPTG诱导下获得特异性表达。
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| 关 键 词： | TOP3 原核表达载体 克隆 |
CONSTRUCTION AND EXPRESSION OF A FUSION TOXIN TOP3 IN PROKARYOTIC EXPRESSION SYSTEM |
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| Liu Fang,Dang Guoquan. CONSTRUCTION AND EXPRESSION OF A FUSION TOXIN TOP3 IN PROKARYOTIC EXPRESSION SYSTEM[J]. Journal of Qinghai Medical College, 2010, 31(3): 154-156,165 |
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| Authors: | Liu Fang Dang Guoquan |
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| Affiliation: | (Dept.of Biochemistry,Qinghai University Medical College) |
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| Abstract: | Objective To construct and express fusion toxin TOP3 in order to develop a targeted toxin that capable of eliminating abnormal cells,for example,cervical carcinoma cells.Methods The expression prokaryotic plasmids pET-28a（＋）-TAT-ODD-CASPASE3（pET-28a（＋）-TOP3） was constructed,and transformed pET-28a（＋）-TOP3 into E.coli BL21（DE3）pLysS strain to be induced by IPTG.The obtained proteins were analyzed by SDS-PAGE.Results The recombinant plasmids were constructed successfully.E.coli transformed with the recombinant plasmid expressed an extra band.Conclusion The present study has successfully constructed prokaryotic expression victor of pET-28a（＋）-TOP3 andgained specific expression induced by IPTG. |
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| Keywords: | TOP3 Prokaryotic expression vector Cloning |
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