COX-2蛋白在鼻咽癌中的表达及其临床意义

目的 探讨COX-2(cyclooxygenase-2)蛋白在鼻咽癌组织中的表达及其临床意义。方法 分别应用免疫组化方法（IHC）和蛋白印迹方法（Western Blot）检测47例鼻咽癌组织（其中用IHC33例，Western Blot14例）及24例鼻咽部炎症组织中COX-2蛋白的表达。结果 COX-2蛋白在鼻咽癌组织的阳性表达率为81.8%（27/33），在鼻咽炎组织的阳性表达率为25%（3/12），两者表达差异有统计学意义（P=0.001）。Western Blot结果显示，在两种组织中COX-2蛋白表达的相对值分别为1.258±0.034和0.487±0.076，在鼻咽癌组表达显著增高（P=0.000）。结论 COX-2蛋白的高度表达在鼻咽癌的发生、发展中起重要作用,选择性COX-2抑制剂有望成为鼻咽癌化疗的重要药物。

Expression and clinical significance of cox-2 protein in nasopharyngeal carcinoma

Objective To investigate expression and its clinical significance of COX-2 protein in nasopharyngeal carcinoma. Methods The immunohistochemical method and Western blot method were used to determine expression of COX-2 protein in specimens of 47 cases (33 cases by IHC and 14 by Western Blot ) of nasopharyngeal carcinoma and 24 cases of normal nasopharyngitis tissues. Results Positive staining rate of COX-2 in specimens of nasopharyngeal carcinoma (81.8%) was significantly higher than that of normal nasopharyngitis tissues (25%)(P=0.001). Western Blot assay revealed that the relative COX-2 protein level was 1.258±0.034 and 0.487±0.076 respectively in the two tissues,which was significantly higher in nasopharyngeal carcinoma(P=0.000). Conclusion Over-expression of COX-2 may play a crucial role in the carcinogenesis and progression of nasopharyngeal carcinoma, selectivity COX-2 inhibiter may become an important chemotherapy drug for nasopharyngeal carcinoma.