Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination |
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| Authors: | Tree Julia A Elmore Michael J Javed Sajid Williams Ann Marsh Philip D |
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| Affiliation: | Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, Wiltshire SP4 0JG, United Kingdom. julia.tree@hpa.org.uk |
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| Abstract: | Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs. |
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