| rhIL-2/GM-CSF融合蛋白抗体制备、鉴定及其生物学活性研究 |
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| 引用本文： | 林来兴妹,周明乾,陈泽洪,张亚莉,王小宁.rhIL-2/GM-CSF融合蛋白抗体制备、鉴定及其生物学活性研究[J].第二军医大学学报,2002,23(10):1103-1105. |
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| 作者姓名： | 林来兴妹 周明乾 陈泽洪 张亚莉 王小宁 |
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| 作者单位： | 第一军医大学分子免疫学研究所,广州,510515 |
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| 基金项目： | 国家 973计划资助项目 (2 0 0 1CB5 10 0 0 8),国家自然科学基金资助项目 (3 9770 692 ),广东省自然科学基金资助项
目 (0 10 613 ) |
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| 摘 要： | 目的：制备rhIL-2/GM-CSF融合蛋白的抗体,并观察该抗体的特异性及对融合蛋白生物学活性的影响。方法：用纯化的融合蛋白（rhIL-2/GM-CSF）作为抗原,免疫新西兰兔后制备抗血清,用ELISA和Dot-ELISA检测抗体滴度和特异性。用依赖株细胞增殖实验检测抗体对rhIL-2/GM-CSF生物学活性的影响。结果：此抗体效价高,特异性好,不仅与融合蛋白（rhIL-2/GM-CSF）、IL-2以及GM-CSF发生反应,而且能够竞争抑制rhIL-2/GM-CSF的生物学活性。结论：此抗体可用于融合蛋白（rhIL-2/GM-CSF）的结构和功能研究。
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| 关 键 词： | rhIL－2 GM－CSF 融合蛋白 抗体 制备 鉴定 生物学活性 白细胞介素－2 粒－巨噬细胞集落刺激因子 |
| 文章编号： | 0258-879X(2002)10-1103-03 |
| 修稿时间： | 2002年8月28日 |
Preparation, identification and biological activity of rhIL-2/GM-CSF fusion protein antibodies |
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| LINLAI Xing Mei,ZHOU Ming Qian,CHEN Ze Hong,ZHANG Ya Li,WANG Xiao Ning.Preparation, identification and biological activity of rhIL-2/GM-CSF fusion protein antibodies[J].Academic Journal of Second Military Medical University,2002,23(10):1103-1105. |
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| Authors: | LINLAI Xing Mei ZHOU Ming Qian CHEN Ze Hong ZHANG Ya Li WANG Xiao Ning |
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| Institution: | LINLAI Xing Mei,ZHOU Ming Qian,CHEN Ze Hong,ZHANG Ya Li,WANG Xiao Ning * |
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| Abstract: | Objective: To prepare and identify recombinant human IL 2/GM CSF(rhIL 2/GM CSF) fusion protein antibodies and to study its specificity and its effect on fusion protein biological activity. Methods: rhIL 2 /GM CSF fusion protein was purified by DEAE Sepharose FF ion exchange chromatography. The purified protein was used to immunize rabbits for the preparation of antisera. The titer and specificity of the antisera were detected by ELISA and Dot ELISA and the biological activity by cell proliferation. Results: The antisera not only reacted with the rhIL 2/GM CSF, IL 2 and GM CSF, but also inhibited the biological activity of the rhIL 2/GM CSF, IL 2 and GM CSF. Conclusion: The obtained antisera can be used to study the structure and function of the rhIL 2/GM CSF. |
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| Keywords: | interleukin 2(IL 2) granulocyte macrophage colony stimulating factor(GM CSF) fusion protein antibody |
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