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| Ku80分子克隆在人肺癌细胞的表达及生物学意义 | |
| 引用本文： | 张捷,张秀娟,杨贵贞,大森繁成,口裕一,栗山乔之.Ku80分子克隆在人肺癌细胞的表达及生物学意义[J].中国免疫学杂志,2001,17(12):657-659. |
| 作者姓名： | 张捷 张秀娟 杨贵贞 大森繁成 口裕一 栗山乔之 |
| 作者单位： | 1. 吉林大学第二医院,长春,130041 2. 吉林大学白求恩医学部免疫学教研室,长春,310021 3. 日本千叶大学医学部,千叶,260 |
| 基金项目： | 此课题已获国家教育部回国人员科研启动基金资助(1999) |
| 摘 要： | 目的：研究Ku80基因分子克隆在人肺癌细胞的表达及生物学意义。方法：用分子克隆的方法构建重组的正义，反义Ku80基因表达质粒，导入人肺癌细胞后，用Northern-blotting法和Western-blotting法证明Ku80正义，反义基因在mRNA和蛋白水平的表达情况。结果：（1）正义或反义Ku80克隆阳性的LCA细胞在mRNA水平均有正义或反义Ku80的表达印迹。（2）仅在正义Ku80克隆阳性的LCA细胞有正义Ku80蛋白表达印迹而反义Ku80克隆阳性的LCA细胞无Ku80蛋白表达印迹，结论：正义或反义Ku80基因的分子克隆可在人肺癌细胞的mRNA得到表达，正义Ku80克隆阳性的LCA细胞有正义Ku80蛋白表达，反义Ku80基因可封闭肿瘤细胞的正义Ku80蛋白的表达，此结论为将Ku基因作为肿瘤靶向基因治疗提供了实验依据。 |
| 关 键 词： | Ku80基因 人肺腺癌细胞 LCA 基因表达 分子克隆 |
| 文章编号： | 1000-484(2001)12-0657-03 |
The expression and its biolgic specification of the Ku80 gene molecular cloning |
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| ZHANG Jie,ZHANG Xiu-Juan,YANG Gui-Zhen et al.The expression and its biolgic specification of the Ku80 gene molecular cloning[J].Chinese Journal of Immunology,2001,17(12):657-659. | |
| Authors: | ZHANG Jie ZHANG Xiu-Juan YANG Gui-Zhen |
| Institution: | ZHANG Jie,ZHANG Xiu-Juan,YANG Gui-Zhen et al.The Second Hospital of the Jilin Univercity,Changchun 130041 |
| Abstract: | Abstract Objective:To study the expression and its biologic specification of the Ku80 gene molecular cloning.Methods: Constructingrecombinant sense or antisense Ku80 expression vectors (Ku80/pCAGGS) and transferring into LCA .Those Ku80 mRNA and protein were de-teited in the Ku80s-LCA, Ku80as-LCA and by Northern-blotting and or Western-blotting.Results: Those Ku80 mRNA were detected in the Ku80s-LCA and Ku80as-LCA by Northern-blotting. Only the sense Ku80 protein was defected in the Ku80s-LCA , but wasn' t detected in the Ku80as-LCA by Western-blotting.Conclusion:The sense or antisense Ku80 were not only transferred in to LCA ,but also expressed in the mR-NA . Only the sense Ku80 protein was expressed in the Ku80s-LCA , but wasn' t expressed in the Ku80as-LCA . The Ku80 is a candidate tar-get for cancer gene therapy. |
| Keywords: | Ku80 gene Human lung carcinoma cell Expression |
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