复方中药提取物对卷烟烟气诱发损伤的保护作用

目的:研究复方中药提取物对卷烟烟气诱发损伤的保护作用.方法:分别检测卷烟烟气对中国仓鼠卵巢细胞(CHO)的细胞毒性、鼠伤寒沙门氏菌诱变性和体外细胞微核率、腺病毒-12/SV40病毒永生化的人支气管上皮细胞(BEP2D)增殖的影响、并检测动物卷烟烟气暴露30 d后外周血细胞、应用氨水引咳实验和酚红排痰实验.观察复方中药提取物以0.03％添加于卷烟后对这些结果的影响.结果:以0.03％添加于卷烟后,复方中药提取物不增加卷烟烟气对鼠伤寒沙门氏菌诱变性及对体外细胞微核的诱导作用,并能抑制卷烟烟气对CHO细胞的细胞毒作用；复方中药提取物还可降低卷烟烟气对人呼吸道上皮细胞的损伤；动物烟气暴露30 d实验中,复方中药提取物添加卷烟2支/d可以逆转对照烟气引起的血红蛋白下降(P＜0.05),4支/d可逆转对照烟气引起的血小板数量的增加(P＜0.05),8支/d可逆转对照烟气引起的动物咳嗽潜伏期降低(P＜0.05),4支/d可逆转对照烟气引起的咳嗽次数增加症状(P＜0.05)、8支/d可逆转对照烟气引起的动物酚红排泌量降低(P＜0.05).结论:复方中药提取物不增加卷烟烟气的体外毒性且有一定减轻毒性的作用或趋势；可以通过减轻吸烟引起的咳嗽、痰液黏稠咳出困难、外周血液学改变等及对呼吸道上皮细胞的直接保护作用减轻卷烟烟气对机体的损伤.

Protective Effect of Chinese Herbal Compound Extract on Damage Induced by Cigarette Mainstream Smoke

Objective: To study the effect of Chinese herbal compound extract on cigarette mainstream smoke induced damage. Method: The effect of cigarette mainstream smoke on cytotoxicity toward Chinese hamster ovarian cells(CHO cells) was detected.Salmonella mutagenicity test, micronucleus test, proliferation of human bronchial epithelial cell line (BEP2D) cells in vitro were also employed,peripheral blood cells, ammonia induced cough, phenolsulfanphthalein excretion test in vivo were carried out after animals were exposed to cigarette smoke for 30 days. Intervention effects of Chinese herb compound on all these tests were evaluated after added in cigarette at 0.03%. Result: After added in cigarette at 0.03%,Chinese herbal compound extract did not increase thesalmonella mutagenicity and micronucleus frequency and could reduce the cytotoxicity toward CHO cells induced by cigarette smoke. Chinese herbal compound extract could reduce the injury of human respiratory epithelial cells. For the research that animals were exposed to cigarette smoke for 30 days, Chinese herbal compound extract (2 cigarette·d^-1) could inhibit the decrease in hemoglobin (P<0.05);4 cigarette·d^-1could inhibit the increase in platelet count and the increasing of times of cough (p<0.05);8 cigarette·d^-1 could reverse the shortenedlatent period of cough (P<0.05),and reverse the decrease of excretion of phenolsulfonphthalein (P<0.05). Conclusion: Chinese herbal compound extract doesn't increase the in vitro cytotoxicity of cigarette smoke and even shows reducing effect or tendency, Chinese herbal compound extract could reduce the injury of cigarette smoke by reducing the cough, thick sputum and change in peripheral blood cells induced by smoking, and though directly protective effect on respiratory epithelial cells.