环氧化酶2和血管内皮生长因子在透明细胞性肾细胞癌中的表达及其与血管生成的关系

目的 探讨透明细胞性肾细胞癌(CCRCC)组织中环氧化酶2(COX-2)和血管内皮生长因子(VEGF)的表达及其与血管生成的关系.方法 应用免疫组化Envision法,检测80例CCRCC和20例正常.肾组织中COX-2和VEGF的表达及CD34标记的微血管密度(MVD),结合临床病理特征进行综合分析,探讨CCRCC组织中COX-2和VEGF的表达与血管生成的关系.结果 在CCRCC组织和正常肾组织中均有COX-2和VEGF表达.80例CCRCC中,COX-2和VEGF表达阳性率分别为65.0%和61.3%,均明显高于正常肾组织(分别为10.0%和20.0%).80例CCRCC组织MVD为70.13±19.99,20例止常肾组织MVD为59.75±15.17,差异有统计学意义(P＜0.05).COX-2的表达与CCRCC组织学分级、TNM分期及淋巴结转移有关(P＜0.05).CCRCC组织中,COX-2的表达与VEGF的表达呈正相关(r=0.485),COX-2和VEGF的表达均与MVD正相关(r分别为0.851和0.736).结论 COX-2与CCRCC血管生成有关,VEGF可能是COX-2调节CCRCC血管生成的重要中介.

Expression of COX-2 and VEGF and their correlation with angiogenesis in human clear cell renal cell carcinoma

Objective To investigate the expression of COX-2 and VEGF in clear cell renal cell carcinoma(CCRCC)and their correlation with tumor angiogenesis.Methods Envision immunohistochemistry was used to determine the expression of COX-2 and VEGF,and microvessel density(MVD)was marked by CD34 in 80 CCRCC tissues and 20 normal kidney tissues.The relationship between the above mentioned markers were analyzed.Results Expressions of COX-2 and VEGF were noted in both CCRCC and normal kidney tissues.The positive rates of COX-2 and VEGF were significantly higher in CCRCC than in normal kidney(P ＜0.05);The expression of COX-2 was correlated with TNM stage(P ＜0.05),histological grade (P ＜0.05)and lymph node metastasis(P ＜0.05)in CCRCC,but not with age(P = 0.663)and diameter of tumor(P =0.528).Both COX-2 expression(r = 0.851,P ＜ 0.01)and VEGF expression(r = 0.736,P ＜ 0.01)were significantly associated with MVD in CCRCC.There was a positive correlation between expression of cox-2 and that of VEGF in CCRCC.Conclusion COX-2 expression is correlated with tumor angiogenesis in CCRCC.It is likely that VEGF is one of the most important mediators in the COX-2 angiogenic pathway.