| 中药何首乌总基因组DNA的提取 |
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| 引用本文： | 严萍,赵树进. 中药何首乌总基因组DNA的提取[J]. 时珍国医国药, 2007, 18(3): 607-609 |
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| 作者姓名： | 严萍 赵树进 |
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| 作者单位： | 1. 华南理工大学生物科学与工程学院,广东,广州,510640
2. 中国人民解放军广州军区广州总医院,广东,广州,510010 |
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| 摘 要： | 目的为获得高质量的何首乌总基因组DNA。方法采用CTAB法提取,对其提取方法进行改进。结果用改进的方法提取的样品DNA的OD260/OD280值在1.630~1.955之间,琼脂糖凝胶上主带清晰、降解较少,能完全被限制性内切酶EcoRⅠ和M seⅠ完全酶切,得到合适的AFLP指纹样式。结论改良的CTAB法提示提取何首乌DNA的理想方法,适合AFLP-PCR反应和其他分子生物学应用。
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| 关 键 词： | 何首乌 DNA提取 AFLP |
| 文章编号： | 1008-0805（2007）03-0607-02 |
| 收稿时间： | 2006-08-14 |
| 修稿时间： | 2006-08-142006-12-10 |
Genomic DNA Extraction from the Polygonum multiflorum Thunb. |
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| YAN Ping,ZHAO Shu-Jin. Genomic DNA Extraction from the Polygonum multiflorum Thunb.[J]. Lishizhen Medicine and Materia Medica Research, 2007, 18(3): 607-609 |
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| Authors: | YAN Ping ZHAO Shu-Jin |
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| Affiliation: | 1. Biological Science Engineering College of South China University of Technology Guangzhou 510640, China; 2. Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China |
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| Abstract: | ObjectiveTo isolate high-quality genomic DNA from Polygonum multiflorum Thunb.MethodsCTAB isolation method was used and modified in this paper.ResultsUsing the modified CTAB methods,the value of OD260/OD280 of DNAs were between 1.630 and 1.955.Strips were clear and less decomposed in agarose electrophoresis.The DNAs were completedly digested with restriction endonucleases and amplified by the polymerase chain reaction(PCR).Sharp fingerprint patterns of AFLP were obtained.ConclusionThe improved CTAB method was optimal for analysis of AFLP-PCR and other molecular biology research. |
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| Keywords: | Polygonum multiflorum Thunb. DNA isolation Amplified fragment length polymorphism |
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