婴幼儿血管瘤iNOS的表达及其与血管内皮细胞增殖能力的关系

目的研究婴幼儿血管瘤组织中诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达,及其与血管内皮细胞增殖能力的关系。方法收集我院2001年1月至2002年5月手术切除的49例血管瘤和29例血管畸形石蜡标本,利用免疫组织化学染色检测其iNOS和Ki-67蛋白的表达。结果49例血管瘤组织中iNOS蛋白的阳性率和Ki-67蛋白的阳性表达指数分别为38%和(10.98±7.93)%,均分别显著高于29例血管畸形组织中的3%和(0.03±0.19)%。增生期血管瘤组Ki-67蛋白的阳性表达指数为(12.67±7.65)%,显著高于消退期血管瘤组的(7.27±7.49)%(P=0.028)。无论是全部78例血管病变还是49例血管瘤,iNOS阳性组的Ki-67蛋白的阳性表达指数均显著高于iNOS阴性组(P<0.05)。结论血管瘤组织中iNOS和Ki-67蛋白的表达均高于血管畸形,而且血管瘤中血管内皮细胞的增殖能力可能与其iNOS阳性率的增高有关。

Relationship between expression of inducible nitric oxide synthase and proliferative potency of endothelium in hemangioma in infants

OBJECTIVE: To investigate relationship between expression of inducible nitric oxide synthase (iNOS) and proliferative potency of endothelium in hemangioma (HM). METHODS: Immunohistochemical staining was used to detect expression of iNOS and Ki-67 protein in 49 cases of HM and 29 cases of vascular malformation (VM). RESULTS: Expressive rate of iNOS and Ki-67 protein of 49 cases of HM was 38% and (10.98 +/- 7.93)%. Expressive rate of iNOS and Ki-67 protein of 29 cases of VM was respectively 3% and (0.03 +/- 0.19)%. The expressive rate of iNOS and Ki-67 protein of HM was significantly higher than that of VM (P = 0.001 and 0). The expressive rate of Ki-67 protein of HM of proliferative phase was (12.67 +/- 7.65)% , which was significantly higher than that of HM of extinctive phase, (7.27 +/- 7.49)% (P = 0.028). CONCLUSIONS: Expression of iNOS and Ki-67 protein of HM is significantly higher than that of VM, and the proliferative potency of HM is significantly higher than that of VM.